Paper - WANFANG MED ONLINE

You Position: Home > Paper

Progress in alternatives for developmental neurotoxicity testing on animals

( views:227, downloads:32 )

Author:: No author available

Journal Title:: Chinese Journal of Pharmacology and Toxicology

Issue:: 1

DOI:: 10.3867/j.issn.1000-3002.2012.01.024

Key Word:: 发育障碍;动物替代实验;模型,神经学;细胞,非哺乳动物

Abstract：胚胎早期暴露于某些工业化学物中,即使是很小剂量,也可导致胚胎脑损伤,引起神经发育性疾病和亚临床脑功能不良.虽然化学物基于动物毒性实验的安全性评价是较可靠的,但这种方法耗时长、成本高,而且不符合目前减少实验动物使用的趋势,因此神经发育毒性(DNT)实验的替代模型逐步引起重视.为建立和完善快速、经济又可高通量筛选受试物的替代方法,本文分别介绍了体外细胞模型和非哺乳动物模型的优势、现阶段应用以及所面临的挑战.这些替代法虽不能完全取代包括哺乳动物在内的体内实验,但它们在区分化合物和识别DNT机制方面将发挥巨大的作用.

Reference

[1]Erwin van Vliet,Cristian Pellizzer,Sandra Coecke,etc.The value of alternative testing for neurotoxicity in the context of regulatory needs[J].2006,21(2).
[2]Yang J,Aschner M.Developmental aspects of blood-brain barrier (BBB) and rat brain endothelial (RBE4) cells as in vitro model for studies on chlorpyrifos transport.[J].2003,24(4).
[3]Hermanson O,Ceccatelli S,Duckworth J,etc.High susceptibility of neural stem cells to methylmercury toxicity: effects on cell survival and neuronal differentiation.[J].2006,97(1).
[4]Tanguay RL,Yang D,La Du J,etc.Chlorpyrifos-oxon disrupts zebrafish axonal growth and motor behavior.[J].2011,121(1).
[5]Andersen HR,Nielsen JB,Grandjean P.Toxicologic evidence of developmental neurotoxicity of environmental chemicals.[J].2000,144(1).
[6]Betts KS.Growing knowledge: using stem cells to study developmental neurotoxicity.[J].2010,118(10).
[7]Trotter J,Simons M.Wrapping it up: the cell biology of myelination.[J].2007,17(5).
[8]Shafer TJ,Radio NM,Breier JM,etc.Assessment of chemical effects on neurite outgrowth in PC12 cells using high content screening.[J].2008,105(1).
[9]Spitzer NC.Electrical activity in early neuronal development[J].2006,444(7120).
[10]Kinsner Ovaskainen A,Hogberg HT,Coecke.mRNA expression is a relevant tool to identify developmental neurotoxicants using an in vitro approach.[J].2010,113(1).
[11]Hogberg HT,Lenas P,van Vliet E,etc.In vitro developmental neurotoxicity (DNT) testing: relevant models and endpoints.[J].2010,31(5).
[12]Bal Price AK,Buzanska,Hogberg HT.Relevance of in vitro neurotoxicity testing for regulatory requirements: challenges to be considered.[J].2010,32(1).
[13]Cowden J,Fan CY,Simmons S.Gene expression changes in developing zebrafish as potential markers for rapid developmental neurotoxicity screening.[J].2010,32(1).
[14]Lin Y,Ton C,Willett C.Zebrafish as a model for developmental neurotoxicity testing.[J].2006,76(7).
[15]Peterson RT,Nass R,Boyd WA,etc.Use of non-mammalian alternative models for neurotoxicological study.[J].2008,29(3).
[16]Costa LG,Weiss DG,Sunol C,etc.Application of in vitro neurotoxicity testing for regulatory purposes: Symposium III summary and research needs.[J].2008,29(3).
[17]Goldberg A,Lein P,Locke P.Meeting report: alternatives for developmental neurotoxicity testing.[J].2007,115(5).
[18]Costa LG,Lasley SM,Ehrich M,etc.Bridging the gap between in vitro and in vivo models for neurotoxicology.[J].1999,51(2).
[19]Powers CM,Seidler FJ,Slotkin TA,etc.Silver exposure in developing zebrafish produces persistent synaptic and behavioral changes.[J].2011,33(2).
[20]Pamela Lein,Ellen Silbergeld,Paul Locke,etc.In vitro and other alternative approaches to developmental neurotoxicity testing (DNT)[J].2005,19(3).
[21]Radio NM,Mundy WR.Developmental neurotoxicity testing in vitro: models for assessing chemical effects on neurite outgrowth.[J].2008,29(3).
[22]Monnet-Tschudi F,Goldberg AM,Allen S,etc.Workgroup report: incorporating in vitro alternative methods for developmental neurotoxicity into international hazard and risk assessment strategies.[J].2007,115(6).
[23]Lilienblum W,Dekant W,Wollin KM,etc.Alternative methods to safety studies in experimental animals: role in the risk assessment of chemicals under the new European Chemicals Legislation (REACH).[J].2008,82(4).
[24]Breier JM,Gassmann K,Kayser.Neural progenitor cells as models for high-throughput screens of developmental neurotoxicity: state of the science.[J].2010,32(1).
[25]Abel J,Moors M,Rockel TD.Human neurospheres as three-dimensional cellular systems for developmental neurotoxicity testing.[J].2009,117(7).
[26]Simonich, M. T.,Tanguay, R. L.,Ye XiaoWei,etc.Toxicity, uptake kinetics and behavior assessment in zebrafish embryos following exposure to perfluorooctanesulphonicacid (PFOS).[J].2010,98(2).
[27]Selderslaghs IW,Hooyberghs J,De Coen W,etc.Locomotor activity in zebrafish embryos: a new method to assess developmental neurotoxicity.[J].2010,32(4).
[28]Salas C,Rodriguez F,Vargas JP,etc.Spatial memory and hippocampal pallium through vertebrate evolution: insights from reptiles and teleost fish.[J].2002,57(3).
[29]Breier JM,Radio NM,Mundy WR,etc.Development of a high-throughput screening assay for chemical effects on proliferation and viability of immortalized human neural progenitor cells.[J].2008,105(1).
[30]Costa LG..Neurotoxicity testing: A discussion of in vitro alternatives[J].1998,106(Suppl 2).
[31]Kuegler, P.B.,Zimmer, B.,Waldmann, T.,etc.Markers of murine embryonic and neural stem cells, neurons and astrocytes: reference points for developmental neurotoxicity testing[J].2010,1(1).