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Protective effect of low concentration sodium nitrite preconditioning on PC12 cells lesioned by high concentration sodium nitrite
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Pharmacology and Toxicology
- Issue:
- 1
- DOI:
- 10.3867/j.issn.1000-3002.2012.01.017
- Key Word:
- 亚硝酸钠;细胞,培养的;细胞保护
Abstract： 目的 探讨低浓度亚硝酸钠( NaNO2)预处理对高浓度亚硝酸钠损伤PC12细胞的保护作用.方法 NaNO2 0.14 mmol·L-1处理PC12细胞24 h,然后用NaNO245 mmol· L-再处理2h制作预处理模型,噻唑蓝(MTT)法检测细胞的存活率,流式细胞术和Hoechst 33258/ PI双染检测细胞凋亡,比色法检测超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量,Western印迹法检测缺氧诱导因子-1α(HIF-1α)和凋亡相关蛋白表达.结果 与NaNO245 mmol·L-1处理组相比,NaNO2 0.14 mmol·L-1预处理+ NaNO245 mmol· L-1组的PC12细胞存活率增加、凋亡减少(P＜0.05)；细胞SOD、CAT活性和GSH-Px含量明显增加,MDA含量明显下降,促凋亡相关蛋白Bax,胱天蛋白酶9,胱天蛋白酶3表达明显下降,凋亡抑制蛋白Bcl-2和HIF-1α表达明显升高(P＜0.05)；加入一氧化氮特异性清除剂c-PTIO可以逆转这种现象(P＜0.05).结论 低浓度NaNO2预处理增加PC12细胞抗氧化能力,拮抗高浓度NaNO2诱导的细胞凋亡,机制与NaNO2还原为一氧化氮和增加HIF-1 α表达有关.
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