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Therapeutic effect of PEG-rhGCSF on rhesus monkeys with radiation-induced neutropenia
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Pharmacology and Toxicology
- Issue:
- 1
- DOI:
- 10.3867/j.issn.1000-3002.2012.01.016
- Key Word:
- 粒细胞集落刺激因子;聚乙二醇;辐射损伤;粒细胞减少症
Abstract： 目的 研究聚乙二醇重组人粒细胞集落刺激因子(PEG-rhGCSF)对猕猴辐射致粒细胞减少症的治疗作用.方法 正常雄性猕猴全身一次性双侧照射60Co γ 3.0 Gy,24 h后一次性sc给予PEG-rhGCSF 30,100和300 μg· kg-1,及连续10 d rhGCSF 10 μg· kg-1.检测50 d内的外周血象和骨髓造血细胞的情况.结果 猕猴接受照射后,外周血白细胞及绝对中性粒细胞(ANC)迅速下降,造成粒细胞减少症.给药后1d,PEG-rhGCSF治疗组均出现一过性ANC动员峰,且峰值与剂量呈正相关.PEG-rhGCSF可延缓ANC达谷时间且可以提高谷水平.与照射模型组的(7.2±2.5)d相比,PEG-rhGCSF 30,100和300 μg· kg -1组抗生素需求天数3.2士2.9,1.4±1.9和(0.2±0.4)d明显缩短(P＜0.05)；与rhGCSF组粒细胞减少症持续时间(28±7)d相比,PEG-rhGCSF 100和300 μg· kg -1组分别为(19±14)d与(7±6)d显著缩短(P＜0.01).连续注射rhGCSF 10 d与单次注射PEG-rhGCSF的药效作用相当.结论 PEG-rhGCSF对猕猴辐射致粒细胞减少症有明显治疗作用,可促进骨髓粒系增殖、分化成熟以及释放,恢复造血功能.治疗作用呈良好量效关系,与rhGCSF相比具有明显长效作用.
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