Inhibitory effect of schisandrin B on cyclooxygenase-2 expression and prostaglandin E2 content in lungs of rats exposed to silica
- Author:
- No author available
- Journal Title:
- Chinese Journal of Pharmacology and Toxicology
- Issue:
- 1
- DOI:
- 10.3867/j.issn.1000-3002.2012.01.014
- Key Word:
- 五味子乙素;二氧化硅;前列腺素E2;环氧合酶-2
Abstract： 目的 探讨五味子乙素( Sch-B)抑制矽肺纤维化的作用及机制.方法 采用一次性气管内注入1 ml二氧化硅(SiO2)混悬液法制备大鼠矽肺模型,染尘后第1天起开始ig给予Sch-B 80 μg·g -1·d-1,连续28 d.分别给予Sch-B后3,7,14和28 d,取肺采用HE染色观察病理变化；酶联免疫吸附试验(ELISA)测定肺组织前列腺素E2( PGE2)含量；免疫组织化学检测肺组织环氧合酶-2(COX-2)蛋白表达；逆转录聚合酶链式反应(RT-PCR)方法检测肺组织COX-2 mRNA的表达.结果 HE染色结果显示,染SiO2组肺损伤明显,早期表现为明显的肺泡炎,大量炎症细胞浸润,后期肺组织以胶原沉积和肺纤维化改变为主.Sch-B组肺泡炎和肺纤维化程度均较同期染SiO2组明显减轻.染SiO2组各时间点大鼠肺组织PGE2含量均显著高于正常对照组(3,7,14和28 d组分别增加了1.2,0.9,1.0和1.1倍)(P＜0.01)；Sch-B干预3,7,14和28 d组PGE2含量均低于染SiO2组,分别显著减少了41.7％,38.1％,36.4％和47.6％(P＜0.01或P＜0.05).染SiO2后,COX-2蛋白表达明显增强,Sch-B干预后COX-2蛋白表达有所减弱.染SiO23,7,14和28 d组大鼠肺内COX-2 mRNA表达均高于正常对照组,分别明显增加了1.3,1.6,1.3和1.7倍(P ＜0.05,P＜0.01)；Sch-B干预3,7,14和28 d组COX-2表达均低于染SiO2组,分别减少了44.4％,46.8％,41.7％和41.5％,差异有统计学意义(P ＜0.05,P＜0.01).结论 Sch-B对染SiO2大鼠肺损伤的保护效应可能与抑制肺组织COX-2表达及PGE2的诱导性合成与释放有关.
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