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Protective effect of catalpol on lactacysin-induced injury in SH-SY5Y cells
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Pharmacology and Toxicology
- Issue:
- 1
- DOI:
- 10.3867/j.issn.1000-3002.2012.01.012
- Key Word:
- 梓醇;SH-SY5Y细胞;细胞凋亡;蛋白酶体;神经保护
Abstract： 目的 探讨梓醇对蛋白酶体抑制剂乳胞素诱导的人神经母细胞瘤(SH-SY5Y)细胞损伤的保护作用及其可能机制.方法 梓醇10 μmol·L-1预处理SH-SY5Y细胞lh后,加入乳胞素10 μmol·L-1继续处理24 h.倒置显微镜下观察细胞形态的变化,MTT比色法检测细胞存活率,流式细胞仪检测细胞凋亡率,Hoechst33258染色观察细胞核形态的变化,酶联免疫吸附检测细胞内20S蛋白酶体含量.结果 与正常对照组相比,梓醇10 μmol·L-1对细胞存活率、形态和凋亡及20S蛋白酶体含量无显著差异；乳胞素10 μmol·L-组细胞存活率为(72.0±1.8)％,明显降低(P＜0.05),细胞凋亡率为(64.7±2.6)％,明显增高(P＜0.05).Hoechst33258染色发现梓醇细胞核形态改变,出现凋亡小体；细胞内20S蛋白酶体含量降低60％,差异具有统计学意义(P＜0.05).与乳胞素10 μmol· L-1组相比,梓醇10μmol· L-1预处理组细胞存活率(87.9±2.2)％明显增高(P＜0.05),细胞凋亡率为(51.4±1.5)％,明显降低(P＜0.05).Hoechst33258染色发现,梓醇细胞核形态明显改善；细胞内20S蛋白酶体含量升高了1.9倍,差异具有统计学意义(P＜0.05).结论 梓醇对乳胞素诱导的SH-SY5Y细胞损伤具有保护作用,其机制可能与梓醇提高SH-SY5Y细胞内20S蛋白酶体含量有关.
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