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Therapeutie effect of Dabuyin Wan on precocious puberty model rats
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Pharmacology and Toxicology
- Issue:
- 1
- DOI:
- 10.3867/j.issn.1000-3002.2012.01.010
- Key Word:
- 大补阴丸;达那唑;青春期,早熟;促性腺激素释放激素;Kiss-1;G蛋白偶联受体54
Abstract： 目的 探讨大补阴丸对真性性早熟大鼠的治疗作用,评价大补阴丸对真性性早熟的疗效并探讨可能的作用机制.方法 5日龄SD雌性大鼠一次性sc给予达那唑30 μg·g-1建立性早熟模型.15日龄时开始ig给予大补阴丸0.81,1.62和3.24 g·kg-1,每天1次,至模型组大鼠阴门开启数超过50％.21日龄起,密切观察各组大鼠阴门开启情况,并记录开启时的日龄.对阴门已开启的大鼠,于每日早晨进行阴道脱落细胞涂片,显微镜下观察性周期的变化.当模型组阴门开启大鼠数超过该组的50％时(33日龄),所有大鼠股动脉放血处死,取子宫和卵巢计算子宫和卵巢系数,并常规制作子宫和卵巢组织切片测定子宫壁厚度和卵巢黄体生成数；半定量逆转录(RT) -PCR法测定下丘脑促性腺激素释放激素(GnRH)、G蛋白偶联受体54( GPR54)和Kiss-1 mRNA的表达水平.结果 大补阴丸3.24 g·kg -组子宫系数为115±12,较模型组154±14显著降低(P＜0.05)；大补阴丸3.24 g·kg-1组子宫壁厚度为(166±27)μm,较模型组(477±71)μm显著降低(P＜0.05),并使卵巢黄体生成个数显著减少；大补阴丸能明显降低下丘脑GnRH,GPR54和Kiss-1mRNA的表达水平,卵巢系数则无明显变化.结论 大补阴丸可能通过抑制下丘脑Kiss-1和GPR54基因表达,抑制下丘脑GnRH的合成和释放,从而抑制下丘脑-垂体-性腺轴的启动,发挥治疗真性性早熟的作用.
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