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Protective effect of compound Ginkgo biloba on alcohol-induced liver injury and its mechanism
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Pharmacology and Toxicology
- Issue:
- 1
- DOI:
- 10.3867/j.issn.1000-3002.2012.01.009
- Key Word:
- 银杏;植物提取物;酒精性肝疾病;细胞色素P450CYP2E1;细胞色素P450CYP3A4
Abstract： 目的 观察银杏叶制剂(CGB)对酒精性肝损伤的防护作用.方法 白酒-玉米油混悬液ig给予大鼠造肝损伤模型,连续10周,造模同时分别ig给予银杏叶提取物(GBE)0.8 g·kg -,CGB 2.4,0.8和0.4g·kg -1或联苯双酯0.15 g·kg-1.取血测定各组大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)及肝匀浆丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽(GSH).并观察肝组织病理及肝细胞线粒体超微结构变化.采用“cocktail”探针药物法测定大鼠ig给予CGB前后血浆中相应的CYP2E1和CYP3 A4酶探针氯唑沙宗和氨苯砜的血药浓度,并计算药代动力学参数.通过药代动力学参数的变化,评价CGB对CYP2E1和CYP3A4酶活性的影响.结果 连续ig给予乙醇后,大鼠血清转氨酶显著高于正常对照组(P＜0.01),与模型组比较,CGB 0.8和2.4 g·kg-1组ALT和AST显著降低(P＜0.05)；CGB 0.8和2.4 g·kg-1组肝匀浆MDA显著降低、GSH和SOD明显升高(P＜0.05).ig给予CGB前,模型组大鼠氯唑沙宗和氨苯砜的AUC0 -24,cmax均较正常对照组显著降低(P＜0.05)；CGB可使正常大鼠血浆氯唑沙宗和氨苯砜AUC0-24下降(P＜0.05),使模型组大鼠血浆氯唑沙宗和氨苯砜的AUC0 -24和cmax显著升高(P＜0.05).结论 CGB对酒精性肝损伤有防护作用,其机制与氧化应激干预相关.
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