N-acetylcysteine (NAC) inhibited pulmonary fibrosis in acute respiratory distress syndrome (ARDS)
- Author:
- No author available
- Journal Title:
- Chinese Critical Care Medicine
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1003-0603.2011.10.009
- Key Word:
- N-乙酰半胱氨酸;急性呼吸窘迫综合征;肺纤维化;氧自由基;脂多糖;N-acetylcysteine;Acute respiratory distress syndrome;Pulmonary fibrosis;Oxidant;Lipopolysaccharide
Abstract： Objective To investigate the effect of NAC treatment on lipopolysaccharide (LPS) treated human embryonic lung fibroblasts (HELF),in regard to oxidant injury and changes in indexes related to pulmonary fibrosis in ARDS.Methods Four groups of cultured HELF were treated with:vehicle,LPS (1 μg/ml),NAC (1 mmol/L)+LPS (1 μg/ml) and dexamethasone (DEX,I μmol/L)+LPS (1 μg/ml) for 24 hours.The content of collagen and the γ-glutamylcysteinylglycine (GSH) in the cells were determined.Results As compared to the control group,the collagen content (μg/mg:78.97士1.79 vs.72.90土1.70)and GSH content (μg/mg:23.27±0.92 vs.26.34土 0.83) in LPS group were significantly (P<O.05) higher and lower,respectively; NAC and DEX both suppressed the effect of LPS on collagen content (72.23士1.35,73.64± 1.89 vs.78.97土 1.79); and GSH content (26.52±0.62,25.85士0.60 vs.23.27士0.92)significantly (P< 0.05 0r P< 0.01) in the treated cells.No significant difference was found between NAC+LPS and DEX+LPS group,either in the content of collagen or GSH.Conclusion NAC can inhibit oxidant injury and pulmonary fibrosis in ARDS.
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