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Effects of scutellarin benzyl ester on the expressions of Bcl-2 and Bax in cardiomyocytes injured by acute hypoxia

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Author:
No author available
Journal Title:
CHINESE CRITICAL CARE MEDICINE
Issue:
6
DOI:
10.3760/cma.j.issn.1003-0603.2011.06.006
Key Word:
灯盏乙素;苄酯衍生物;心肌缺氧;Bcl-2;Bax;Scutellarin;Benzyl ester;Myocardial hypoxia;Bcl-2;Bax

Abstract: Objective To investigate the effects of scutellarin benzyl ester on the expressions of Bcl-2 and Bax in cardiomyocytes after acute injury of hypoxia.Methods Cardiomyocytes harvested from neonatal Sprague-Dawley (SD) rats were cultured for 4 days.The cells of the primary culture were assigned randomly to five groups:control group,hypoxia model group (acute hypoxic injury was induced by exposing cells to anoxic condition with 95% N2 and 5% CO2) and high,moderate and low dose of scutellarin benzyl ester groups (pretreated with 100,50 and 25 μmol/L scutellarin benzyl ester before hypoxia) with 6 wells in each group.After hypoxic injury for 6 hours,the activity of lactate dehydrogenase (LDH) and content of nitric oxide (NO) in media were measured by biochemical methods.The mRNA and protein expressions of Bcl-2 and Bax were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting,respectively.Results After hypoxic injury,comparing with those in control group,the leakage of LDH (U/L) in hypoxia model group increased (288.10±30.69 vs.78.75±12.85) and the release of NO (μmol/L)decreased (9.02±1.55 vs.24.11±2.04,both P<0.01).However,high,moderate and low dose of scutellarin benzyl ester could significantly reduce the LDH leakage(64.19±22.90,101.35±22.71,141.34±23.08) and high and moderate dose of scutellarin benzyl ester could increase the NO release (14.45±3.57,12.38±1.57),with remarkably significant statistical difference(all P<0.01).After hypoxic injury,comparing with those in control group,the mRNA and protein expressions of Bcl-2 in hypoxia model group decreased(Bcl-2 mRNA:0.544±0.108 vs.0.837±0.213,Bcl-2 protein:0.813±0.041 vs.0.917±0.046),and the mRNA and protein expressions of Bax in hypoxia model group increased (Bax mRNA:0.918±0.228 vs.0.493±0.123,Bax protein:0.623±0.031 vs.0.490±0.025,all P<0.01).Comparing with those in hypoxia model group,high and moderate dose of scutellarin benzyl ester could up-regulate the mRNA and protein expressions of Bcl-2(Bcl-2 mRNA:0.708±0.123,0.604±0.116 vs.0.544±0.108,Bcl-2 protein:0.887±0.044,0.850±0.043 vs.0.813±0.041) and down-regulate the mRNA and protein expressions of Bax (Bax mRNA:0.614±0.136,0.728±0.152 vs.0.918±0.228,Bax protein:0.518±0.026,0.547±0.027 vs.0.623±0.031,all P<0.01).However,there was rio statistical difference in the mRNA and protein expressions of Bcl-2 and Bax between hypoxia model group and low dose of scutellarin benzyl ester group (Bcl-2 mRNA 0.551±0.112,Bcl-2 protein 0.823±0.041;Bax mRNA 0.857±0.218,Bax protein 0.617±0.031).Conelusion Scutellarin benzyl ester has a protective effect on acute myocardial hypoxic injury,and the mechanism is probably related to the regulation of the expressions of Bcl-2 and Bax.

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