Abstract： Objective To observe the changes in membrane pump activity of kindey,myocardium and pancreas in rabbits with acute renal failure(ARF) in rabbits,and inquire into the mechanism of multiple organ injury subsequent to ARF. Methods Forty-two rabbits were randomly divided into control group,HgCl2 group and glycerine group,and the latter two groups were subdivided into 12,24,48-hour subgroups,with 6 rabbits in each group.The ARF model was reproduced by hypodermic injection 1% HgCl2(1.3 ml/kg) in HgCl2 group,or intramuscularly injection 50% glycerine(10 ml/kg) in glycerine group,respectively.At different time points,the kindey,myocardium and pancreas were harvested,and homogenates of them were prepared.The ATPase activities of different organ homogenates were determined. Results It showed that the activities of Na+-K+-ATPase,Ca2+-ATPase,Ca2+-Mg2+-ATPase of renal homogenate in two model groups were reduced gradually with worsening of renal function,and they became lowest at 48 hours[(0.84±0.16),(0.52±0.17),(0.45±0.09) μmol·mg-1·h-1 in HgCl2 group;(0.85±0.22),(0.49±0.21),(0.54±0.17) μmol·mg-1·h-1 in glycerine group].The respective activities of Na+-K+-ATPase,Ca2+-ATPase,Mg2+-ATPase,Ca2+-Mg2+-ATPase of myocardium and pancreas homogenates in two model groups were reduced gradually following depression of renal function,and they became lowest at 48 hours[(0.56±0.11),(0.51±0.19),(0.55±0.19),(0.37±0.19) μmol·mg-1·h-1 in HgCl2 group and(0.52±0.19),(0.62±0.10),(0.61±0.16),(0.54±0.10) μmol·mg-1·h-1 in myocardium homogenate of glycerine group;(0.81±0.12),(0.71±0.15),(0.73±0.18),(0.62±0.16) μmol·mg-1·h-1 in HgCl2 group and(0.72±0.13),(0.57±0.18),(0.66±0.14),(0.59±0.23) μmol·mg-1·h-1 in pancreas homogenate of glycerine group],there was statistical difference compared with control group(P<0.05 or P<0.01). Conclusion The mechanism of myocardial and pancreatic injury subsequent to ARF might be related to reduction of the activity of cell membrane pump.