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The role of expression of Toll-like receptor 2 and Toll-like receptor 4 in hyperoxia-induced acute lung injury in rat

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Author:
No author available
Journal Title:
CHINESE CRITICAL CARE MEDICINE
Issue:
11
DOI:
10.3760/cma.j.issn.10030603.2009.11.002
Key Word:
高浓度氧;肺损伤;急性;Toll样受体;hyperoxia;acute lung injury;Toll-like recepter

Abstract: Objective To investigate the role of Toll-like receptors (TLRs) in pathogenesis of hyperoxia-induced acute lung injury (ALI). Methods Thirty-two Sprague-Dawley (SD) rats were randomly divided into air control group,hyperoxia for 24 hours group,hyperoxia for 48 hours group,and hyperoxia for 72 hours group,with 8 rats in each group. At corresponding exposure time points the animals were sacrificed,then the left lung was removed and measured the wet/dry weight (W/D) ratio. The severity of lung injury was assessed by lung histopathology scores,the expression of TLR2 mRNA and TLR4 mRNA in lung tissue was assessed by real-time reverse transcription-polymerase chain reaction (RT-PCR),the expression of TLR2 and TLR4 protein in lung tissue was measured by Western blotting,the amount of interleukin-6 (IL-6) in lung tissue was measured by enzyme linked immunosorbent assay (ELISA). Results After hyperoxia exposure,an increase in lung W/D ratio was noted compared with that of the air control group (all P<0.01). Lung injury scores in hyperoxia for 48 hours (2.69±0.52) and 72 hours group (3.94±0.62) were significantly higher than that of the air control group (0.41±0.38,both P<0.01). Real-time RT-PCR results showed that TLR2 mRNA and TLR4 mRNA expression in lung tissue in hyperoxia exposure groups was significantly higher than that of the air control group (0.67±0.15,0.63±0.19),and it reached the peak at 24 hours (1.82±0.33,1.35±0.26,both P<0.05). Western blotting study showed increased protein expression of TLR2 and TLR4 in lung tissues of the hyperoxia groups compared with the air control group [(7.20±0.51)%,(14.26±0.19)%],and it peaked at 48 hours and 72 hours [(28.12±0.24)%,(81.35±0.82)%,both P<0.05]. ELISA assay also demonstrated upregulation of IL-6 levels in lung tissue of hyperoxia groups compared with the air control group [(639.38±95.24) pg/L],and it reached the peak at 72 hours [(1 300.58±442.24) pg/L,P<0.05]. Conclusion The prolonged exposure to hyperoxia may cause ALI in rat,and TLR2 and TLR4 plays an important role in the development of hyperoxia-induced ALI in rat.

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