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Effect of arginine vasopressin on vascular reactivity and calcium sensitivity of vascular smooth muscle and its relationship to protein kinase C following hemorrhagic shock in rats
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 3
- DOI:
- 10.3321/j.issn:1003-0603.2008.03.004
- Key Word:
- 失血性休克;精氨酸血管加压素;蛋白激酶;血管反应性;钙敏感性
Abstract： 目的 探讨精氨酸血管加压素(AVP)对失血性休克大鼠血管反应性和钙敏感性的恢复作用与蛋白激酶C(PKC)亚型的关系.方法 取失血性休克大鼠肠系膜上动脉(SMA)并去除内皮,利用离体血管环张力测定技术,观察了AVP(5×10-11、5×10-10和5×10-9 mol/L)调节对休克SMA对去甲肾上腺素(NE)反应性和钙敏感性的作用及其与PKCα、δ亚型的关系.结果 AVP(5×10-11、5×10-10和5×10-9 mol/L)可明显恢复休克后的血管反应性和钙敏感性,使SMA对NE和Ca2+的量一效曲线明显左移,血管环产生的最大收缩张力(Emax)升高(P均＜0.01),且呈一定的剂量依赖关系,各剂量组间比较差异均有统计学意义(P均＜0.05).而特异性的PKCα、δ亚型抑制剂G66976(5×10-6 mol/L)和Rottlerin(10-5 mol/L)均可拮抗AVP(5×10-10 mol/L)诱导的休克后血管反应性和钙敏感性升高,抵消了AVP诱导的NE和Ca2+的量一效曲线左移,使NE的Emax明显降低(P＜0.05或P＜0.01).结论 AVP能剂量依赖性地升高失血性休克大鼠的血管反应性和血管平滑肌钙敏感性,其机制可能与PKCα、δ亚型激活有关.
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