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Screening for cellular signal transduction pathway involved in C-reactive protein induced endothelial cell inflammation
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 12
- DOI:
- 10.3760/j.issn:1003-0603.2007.12.002
- Key Word:
- C-反应蛋白;内皮细胞;炎症;信号转导;通路
Abstract： 目的 探讨C-反应蛋白(CRP)作为急性期反应蛋白参与内皮系统炎症损伤过程涉及的信号通路及其意义.方法 采用人脐静脉内皮细胞(HUVEC)进行培养、鉴定,实验用第五代细胞,给予20 mg/L的CRP处理24 h,设立对照,提取RNA,进行信号转导通路特异性基因芯片检测.结果 CRP处理组分别有13种基因表达增加、25种基因表达降低,其中炎症生长因子相关基因WISP2表达增加37.63倍,诱导凋亡关键信号通路p53相关基因表达增加30.50倍,而抑制凋亡相关信号通路Bcl-x和Bcl-2相关基因表达降低9.61%和49.95%.CRP处理后血管细胞黏附分子(VCAM)相关基因(VCAM-1)表达增加2.75倍,而血清细胞间黏附分子(ICAM)相关基因表达两组差异无统计学意义.结论 CRP可能参与了内皮系统的炎症发生,其机制可能是通过诱导细胞凋亡及激活细胞黏附相关蛋白信号通路的表达实现的.
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