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Effects of endothelial lipase on mRNA expression of adhesion molecule of human umbilical vascular endothelial cells
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 12
- DOI:
- 10.3760/j.issn:1003-0603.2007.12.001
- Key Word:
- 内皮脂酶;人脐静脉内皮细胞;黏附分子;肿瘤坏死因子-α
Abstract： 目的 研究人脐静脉内皮细胞(HUVECs)表达黏附分子与内皮脂酶(EL)的关系,以及EL对内皮细胞黏附分子表达的影响.方法 以肿瘤坏死因子-α(TNF-α)10 μg/L刺激HUVECs,采用逆转录-聚合酶链反应(RT-PCR)检测细胞间黏附分子-1(ICAM-1)、血管内皮黏附分子-1(VCAM-1)和E-选择素的mRNA表达变化,以50 μg/L的抗EL抗体进行预处理,再检测黏附分子mRNA表达的变化.利用凝胶成像系统拍照、分析、计算黏附分子产物相对含量.结果 TNF-α作用于HUVECs后,黏附分子ICAM-1、VCAM-1和E-选择素的mRNA表达均增高,与正常对照组比较差异有统计学意义(P均＜0.01);该作用可被50 μg/L的抗EL抗体所拮抗(P＜0.05或P＜0.01).结论 EL参与了内皮细胞黏附分子表达的调控,推测它可能通过影响内皮细胞黏附分子表达而参与动脉粥样硬化的病理生理过程.
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