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Protective effect of N-acetyl-L-cysteine on liver and lung injury in mice after partial hepatic ischemia/reperfusion
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 7
- DOI:
- 10.3760/j.issn:1003-0603.2007.07.004
- Key Word:
- N-乙酰半胱氨酸;缺血/再灌注损伤,肝;肝损伤;肺损伤;Toll样受体
Abstract： 目的 研究抗氧化剂N 乙酰半胱氨酸(NAC)对肝脏部分缺血/再灌注(I/R)后肝、肺损伤的保护作用及对Toll样受体2/4(TLR2/4)表达的影响.方法 以BALB/c小鼠复制肝脏部分I/R损伤模型,将小鼠随机分为假手术组、I/R组和NAC干预组(NAC组),每组10只.于再灌注后1 h和3 h取门静脉血测定血清肿瘤坏死因子-α(TNF-α)浓度,取眼动脉血测定血浆丙氨酸转氨酶(ALT)水平;同时取受损肝、肺组织行逆转录-聚合酶链反应(RT-PCR)和蛋白质免疫印迹法(Western blotting)观察TLR2/4表达的变化.结果 I/R后受损肝叶和肺组织内出现TLR2/4 mRNA和蛋白的高表达,NAC干预可抑制其表达水平(P＜0.05或P＜0.01).I/R损伤后血中TNFα和ALT水平均较假手术组明显升高;NAC干预后各时间点TNF-α和ALT均较I/R组明显下降(P＜0.05或P＜0.01).结论 小鼠肝部分I/R后,TLR2/4不仅在受损肝组织内有表达,在远隔器官肺内也有表达.NAC可通过调整氧化还原状态,抑制再灌注后TLR2/4的活化和细胞因子TNFα的表达,从而减轻肝、肺组织的损伤.
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