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Role of polymorphonuclear neutrophil apoptosis and expression of Fas and caspase-3 in the systemic inflammatory response syndrome
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1003-0603.2007.01.004
- Key Word:
- 全身炎症反应综合征;中性粒细胞;细胞凋亡;基因表达
Abstract： 目的 观察全身炎症反应综合征(SIRS)时中性粒细胞(PMN)凋亡的异常变化及其凋亡调控基因的表达情况,评价其临床意义.方法 8例SIRS患者(均为急性胰腺炎患者)作为SIRS组,6名健康献血者作为正常对照组.分别观察两组外周血PMN凋亡率、凋亡调控基因Fas/FasL和凋亡信号转导分子天冬氨酸特异性半胱氨酸蛋白酶-3(caspase-3)及血清白细胞介素-6(IL-6)、IL-8的水平.结果 SIRS组外周血血清IL-6和IL-8水平均明显高于正常对照组(P均＜0.01).SIRS组患者外周血PMN细胞凋亡率较正常对照组明显减少(P＜0.01);两组PMN在体外培养24 h后,用蛋白质免疫印迹法(Western blotting)没有检测到FasL表达;SIRS组患者外周血PMN的Fas、caspase-3表达水平与正常对照组比较明显降低(P均＜0.01).结论 SIRS患者存在PMN的凋亡异常和Fas、caspase-3表达水平下调,PMN凋亡延迟在SIRS的发生发展中有着重要意义.
- [1]张新日,杜永成,姜宏英,等.中性粒细胞活化在呼吸机所致肺损伤中的作用[J].2005,(6).
- [2]隰建成,周宝桐,蒋礼先,等.创伤性脓毒症过程中外周血中性粒细胞凋亡的实验研究[J].2005,(9).
- [3].American College of Chest Physicians/Society of Critical Care Medicine Consensus Conference: definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis.[J].1992,20(6).
- [4]Mitchell Levy,Mitchell Fink,John Marshall,etc.2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference[J].2003,31(4).
- [5]M F, Jimenez,R W, Watson,J, Parodo,etc.Dysregulated expression of neutrophil apoptosis in the systemic inflammatory response syndrome.[J].1997,132(12).
- [6]D. L. Vaux,A. Strasser.The molecular biology of apoptosis[J].1996,93(6).
- [7]Clement A Diglio,Jason S Taub,David M Liberati,etc.Intestinal Epithelial Cells Modulate PMN Activation and Apoptosis Following Bacterial and Hypoxic Challenges[J].2005,58(6).
- [8]R C, Bone.Sir Isaac Newton, sepsis, SIRS, and CARS.[J].1996,24(7).
- [9]Aruffo A,Ledbetter JA,Kiener PA,etc.Differential expression of Fas (CD95) and Fas ligand on normal human phagocytes: implications for the regulation of apoptosis in neutrophils.[J].1996,184(2).
- [10]Song-Chou Hsieh,Ming-Ho Huang,Chang-Youh Tsai,etc.The Expression of Genes Modulating Programmed Cell Death in Normal Human Polymorphonuclear Neutrophils[J].1997,233(3).
- [11]Keel M,Harter L,Trentz O,etc.Endotoxin reduces CD95-induced neutrophil apoptosis by cIAP-2-mediated caspase-3 degradation.[J].2004,199(4).
- [12]Rostagno P,Rossi B,Le'Negrate G,etc.Downregulation of caspases and Fas ligand expression, and increased lifespan of neutrophils after transmigration across intestinal epithelium.[J].2003,10(2).
- [13]Wang JH,Kell MR,Wu QD,etc.Bacterial Lipoprotein Delays Apoptosis in Human Neutrophils through Inhibition of Caspase-3 Activity: Regulatory Roles for CD14 and TLR-2.[J].2004,173(8).