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Primary investigaion of the relationship between glucocorticoid induced leucine zipper and inflammatory reaction
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1003-0603.2007.01.003
- Key Word:
- 糖皮质激素;地塞米松;亮氨酸拉链蛋白;核转录因子-κB;激活蛋白-1;全身炎症反应综合征
Abstract： 目的 探讨糖皮质激素诱导的亮氨酸拉链蛋白(GILZ)在炎症反应过程中的作用机制.方法 采用无血清RPMI1640培养基分两组培养人单核细胞株THP-1细胞,其中刺激组加入地塞米松(DEX)刺激,而对照组仅加无血清RPMI1640培养基.12 h后提取两组细胞总RNA和总蛋白;用逆转录-聚合酶链反应(RT-PCR)方法在转录水平半定量检测GILZ基因;用蛋白质免疫印迹法(Western blotting)在翻译水平检测核转录因子-κB(NF-κB)p65与激活蛋白-1(AP-1)的蛋白表达.收集10例临床创伤患者[创伤严重度评分(ISS)≥16分]伤后24 h内的外周血,提取白细胞后分为刺激组和对照组,对照组给予无血清RPMI1640培养基,刺激组在对照组的基础上加入DEX刺激,培养12 h后提取白细胞总RNA和总蛋白;用RT-PCR方法在转录水平半定量检测GILZ基因;用Western blotting方法在翻译水平检测NF-κB p65与AP-1的蛋白表达.结果 在DEX刺激下,DEX刺激组THP-1细胞和创伤患者外周血白细胞GILZ mRNA表达水平均较对照组升高(P均＜0.01);DEX刺激组NF-κB p65与AP-1蛋白在THP-1细胞和创伤患者外周血白细胞的表达水平均低于对照组(P均＜0.01).结论 糖皮质激素可上调GILZ基因表达;GILZ的高表达可以抑制NF-κB与AP1的活性,具有抗炎症反应的作用.
- [1]林洪远,盛志勇.全身炎症反应和MODS认识的变化及现状[J].2001,(11).
- [2]林季,颜光涛.急性炎症反应中真核细胞转录因子-κB的信号转导作用[J].2003,(1).
- [3]蔺宏伟,岳茂兴.多器官功能障碍综合征与免疫失衡[J].2001,(9).
- [4]Christman JW,Blackwell TS,Lancaster LH.Nuclear factor kappa B: a pivotal role in the systemic inflammatory response syndrome and new target for therapy (see comments)[J].1998,24(11).
- [5]Mittelstadt PR,Ashwell JD.Inhibition of AP-1 by the glucocorticoid-inducible protein GILZ.[J].2001,276(31).
- [6]Cannarile L,Tabilio A,D'Adamio F,etc.Cloning, chromosomal assignment and tissue distribution of human GILZ, a glucocorticoid hormone-induced gene.[J].2001,8(2).
- [7]Balogh J,Finlay Jones-JJ,Bonder CS,etc.Differential responses of human monocytes and macrophages to IL-4 and IL-13.[J].1999,66(4).
- [8]C, Riccardi,M G, Cifone,G, Migliorati.Glucocorticoid hormone-induced modulation of gene expression and regulation of T-cell death: role of GITR and GILZ, two dexamethasone-induced genes.[J].1999,6(12).
- [9]Francesca D'Adamio,Ornella Zollo,Rosalba Moraca,etc.A New Dexamethasone-Induced Gene of the Leucine Zipper Family Protects T Lymphocytes from TCR/CD3-Activated Cell Death[J].1997,7(6).
- [10]E, Ayroldi,G, Migliorati,S, Bruscoli,etc.Modulation of T-cell activation by the glucocorticoid-induced leucine zipper factor via inhibition of nuclear factor kappaB.[J].2001,98(3).
- [11]Dominique, Berrebi,Stefano, Bruscoli,Nicolas, Cohen,etc.Synthesis of glucocorticoid-induced leucine zipper (GILZ) by macrophages: an anti-inflammatory and immunosuppressive mechanism shared by glucocorticoids and IL-10.[J].2003,101(2).
- [12]Jugnu Jain,Christine Loh,Anjana Rao.Transcriptional regulation of the IL-2 gene[J].1995,7(3).
- [13]Y, Yang,M, Mer?ep,C F, Ware,etc.Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids.[J].1995,181(5).
- [14]Zollo O,Marchetti C,Ayroldi E,etc.Glucocorticoid-induced leucine zipper inhibits the raf-extracellular signal-regulated kinase pathway by binding to raf-1.[J].2002,22(22).
- [15]Brun Buisson-C.The epidemiology of the systemic inflammatory response.[J].2000,26(Suppl.1).
- [16]McIntire LV,Lu CM,Russell CG,etc.DNA microarray reveals changes in gene expression of shear stressed human umbilical vein endothelial cells.[J].2001,98(16).
- [17]R C, Bone.Sir Isaac Newton, sepsis, SIRS, and CARS.[J].1996,24(7).