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Effect of ubiquitin-proteasome pathway on inflammatory reaction in intestine and its barrier function in rats with postburn sepsis
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1003-0603.2006.11.006
- Key Word:
- 脓毒症;泛素;核转录因子-κB;肿瘤坏死因子-α;烧伤
Abstract： 目的 研究泛素蛋白酶体途径对烫伤脓毒症大鼠肠组织核转录因子-κB(NF-κB)活化、肿瘤坏死因子-α(TNF-α)表达以及血浆二胺氧化酶(DAO)活性的作用.方法 采用30%总体表面积(TBSA)Ⅲ度烫伤合并内毒素攻击大鼠为模型模拟临床烫伤脓毒症.60只Wistar大鼠随机分为正常对照组、烫伤脓毒症模型组、泛素-蛋白酶体抑制剂N-乙酰亮氨酰亮氨酰正亮氨酸(ALLN)组、NF-κB抑制剂吡咯烷二硫基甲酸酯(PDTC)组.采用凝胶电泳迁移率改变分析法(EMSA)分析肠组织NFκB活性;采用酶联免疫吸附法(ELISA)检测肠组织TNF-α含量;采用分光光度法检测血浆DAO活性.结果 各组肠组织NF-κB活性于伤后1 h均明显增强,并达到高峰(P均＜0.01),之后呈现下降趋势;两种抑制剂均可显著降低伤后1 h和2 h NF-κB的活性.ALLN可明显降低伤后1 h肠组织中TNF-α含量(P＜0.01).两种抑制剂对伤后1 h和2 h血浆DAO活性均无明显影响.结论 早期使用泛素-蛋白酶体抑制剂可降低烫伤脓毒症大鼠肠组织NF-κB活性,降低肠组织中炎症反应,但对肠组织屏障功能无保护作用.
- [1]黎君友,孙丹,吕艺,等.脓毒症大鼠小肠功能变化的研究[J].2004,(6).
- [2]姚咏明,盛志勇.脓毒症信号转导机制的现代认识[J].2003,(1).
- [3]王勇,黄文华.一种改进的核转录因子的电泳迁移率改变分析法[J].2001,(1).
- [4]姚咏明,盛志勇.重视对脓毒症本质的探讨[J].2005,(3).
- [5]黎君友,于燕,郝军,等.分光光度法测定血和小肠组织二胺氧化酶活性[J].1996,(4).
- [6]Maria A, Brown,W Keith, Jones.NF-kappaB action in sepsis: the innate immune system and the heart.[J].2004,9.
- [7]Basilia, Zingarelli.Nuclear factor-kappaB.[J].2005,33(12 Suppl).
- [8]Zen K.,Eunson T.,Yee E.,etc.Lipopolysaccharide-induced NF-kappa B activation in human endothelial cells involves degradation of I kappa B alpha but not I kappa B beta(1)[J].1998,243(2).
- [9]Fei Chen,Yongju Lu,Douglas C. Kuhn,etc.Calpain Contributes to Silica-Induced IκB-α Degradation and Nuclear Factor-κB Activation[J].1997,342(2).
- [10]Stern DM,Martin E,Bierhaus A,etc.Role of NFkappaB in the mortality of sepsis.[J].1997,100(5).
- [11]F, Deryckere,F, Gannon.A one-hour minipreparation technique for extraction of DNA-binding proteins from animal tissues.[J].1994,16.