You Position: Home > Paper
Effect of Rho kinase on hypoxia stimulated proliferation of pulmonary arterial smooth muscle cells
( views:256, downloads:4 )
- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 8
- DOI:
- 10.3760/j.issn:1003-0603.2006.08.002
- Key Word:
- 缺氧;肺动脉;平滑肌细胞;细胞增殖;Rho激酶
Abstract： 目的研究低氧条件下Rho激酶是否参与低氧引起的血管平滑肌细胞增生.方法组织块法培养大鼠肺动脉平滑肌细胞(PASMCs).应用甲基噻唑基四唑(MTT)比色法观察细胞增生情况;流式细胞仪观察细胞周期的变化;蛋白质免疫印迹法(Western blot)检测Rho激酶的表达.结果缺氧组MTT比色吸光度(A)值显著高于常氧组,缺氧24 h+Rho激酶抑制剂Y27632组A值显著低于缺氧24 h组;缺氧组细胞周期G2/M期细胞比例显著高于常氧24 h组,缺氧24 h+Y27632组细胞比例显著低于缺氧24 h组.Western blot结果表明各缺氧组Rho激酶表达均明显高于常氧组,其中以缺氧24 h组表达增高最明显;Rho激酶在缺氧24 h+Y27632组明显低于缺氧24 h组.结论缺氧可诱导PASMCs增殖,缺氧诱导的增生PASMCs中Rho激酶活性水平增加,提示缺氧致PASMCs增殖过程中Rho激酶可能发挥重要作用.
- [1]姚伟,钱桂生,杨晓静,等.小牛血清和血小板源性生长因子对肺动脉平滑肌细胞Na+/H+交换器活性和细胞增殖的影响[J].2001,(12).
- [2]T M, Seasholtz,M, Majumdar,D D, Kaplan,etc.Rho and Rho kinase mediate thrombin-stimulated vascular smooth muscle cell DNA synthesis and migration.[J].1999,84.
- [3]Numaguchi K,Inagami T,Yamakawa T,etc.Involvement of Rho-kinase in angiotensin II-induced hypertrophy of rat vascular smooth muscle cells.[J].2000,35(1 Pt.2).
- [4]McMurtry IF,Bauer NR,Nagaoka T,etc.Hypoxia and Rho/Rho-kinase signaling. Lung development versus hypoxic pulmonary hypertension.[J].2003,543(0).
- [5]Yoshioka K,Saruta T,Hasegawa K,etc.Role of Rho-kinase and p27 in angiotensin II-induced vascular injury.[J].2005,45(4).