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Changes in electroencephalograph and somatosensory evoked potential and their relationship with neuron apoptosis in rat after ischemic insult to brain
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1003-0603.2006.05.004
- Key Word:
- 脑缺血;脑电图;诱发电位,躯体感觉;细胞凋亡
Abstract： 目的探讨大鼠全脑缺血/再灌注(I/R)后体感诱发电位(SEP)和脑电图波谱特征的变化及其与神经元分布的关系.方法SD大鼠35只,采用随机数字表法分为正常组、假手术组、全脑I/R3、12、24、48和72 h组.采用四血管夹闭方法建立全脑I/R模型,末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法(TUNEL)及吖啶橙/溴化乙锭(AO/EB)荧光比色法检测神经元凋亡;记录I/R后不同时间脑电图和SEP的变化,观察海马、额叶皮质和顶叶皮质神经元凋亡数量.结果脑缺血后大鼠脑电图波幅较假手术组显著下降(P均<0.05),δ波构成比明显增加(P均<0.05);伤后SEP的P1波波峰潜伏期较假手术组显著延长(P均<0.05),P1～N1波波幅显著下降(P均<0.05);这些变化与伤后神经元凋亡以及自海马逐渐向额叶皮质和顶叶皮质扩展的结果相吻合.结论脑电图和SEP结合分析可以反映脑缺血损伤后神经元凋亡的发生过程,对临床脑缺血患者的病情和预后判断有参考价值.
- [1]蒋祝昌,毕桂南,石胜良.黄皮酰胺对高血压局灶性脑缺血-再灌注大鼠Bcl-2蛋白表达和细胞凋亡的影响[J].2005,(5).
- [2]刘其强,白宏英,张红,等.大鼠全脑缺血模型的脑电图验证[J].2001,(4).
- [3]陈燕启,刘德红,杨光田.葛根素在大鼠全脑缺血-再灌注时对核因子-κB表达的影响[J].2004,(1).
- [4]汪长胜,霍正禄,杨瑞和,等.沙土鼠短暂性脑缺血后海马CA1区细胞凋亡及亚低温的影响[J].2000,(12).
- [5]章翔,李兵,张志文,等.全脑缺血再灌注大鼠不同时相海马CA1区Bcl-2、Bcl-xL及Bax蛋白的表达[J].2001,(8).
- [6]Heiss W. D..Flow thresholds of functional and morphological damage of brain tissue[J].1983.
- [7]Miragoli L,Frigeni V racco.it,Lorusso V,etc.Comparative study between gadobenate dimeglumine and gadobutrol in rats with brain ischemia: evaluation of somatosensory evoked potentials.[J].2001,36(10).
- [8]Frigeni V racco.it,Miragoli L,Grotti A,etc.Neurotolerability of contrast agents in rats with brain ischemia induced by transient middle cerebral artery occlusion: EEG evaluation.[J].2001,36(1).
- [9]Melgar MA,Gloss D,Rafols J,etc.Postischemic reperfusion: ultrastructural blood-brain barrier and hemodynamic correlative changes in an awake model of transient forebrain ischemia.[J].2005,56(3).