Effect of growth hormone on acute lung injury
- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.1003-0603.2005.09.004
- Key Word:
- 肺损伤,急性;生长激素;内毒素;核转录因子-κB;核转录因子-κB抑制蛋白
Abstract： 目的探讨应激反应阶段生长激素(GH)对脂多糖(LPS)诱导急性肺损伤(ALI)的影响及其机制.方法 112只雄性SD大鼠随机均分为ALI组和GH组,按致伤与否及致伤后的观察时间又随机均分为0、0.5、1、2、4、6和24 h 7个亚组.分别测定大鼠肺泡隔面积密度(PASAD),肺泡隔中性粒细胞数,肺局部肿瘤坏死因子(TNF)和白细胞介素-6(IL-6)水平,肺组织核转录因子-κB(NF-κB)阳性细胞数和肺匀浆液中NFκB抑制蛋白(I-κBα)含量.结果致伤后ALI组大鼠PASAD进行性增大,肺泡隔中性粒细胞数进行性增多,两者均于6 h达峰值,24 h基本恢复正常;GH组大鼠PASAD较ALI组同时间点进一步增大,中性粒细胞数增多更明显.ALI组致伤后0.5 h肺局部TNF水平开始迅速升高,1 h达峰值,其后维持在较高水平,6 h后逐渐恢复;致伤后1 h IL-6水平开始明显升高,4 h达峰值,6 h后逐渐恢复;GH组大鼠IL-6水平升高更明显.ALI组致伤后0.5 h肺组织中NF-κB阳性细胞数明显增多,4 h达峰值,6 h开始恢复;GH组大鼠肺组织NF-κB阳性细胞数明显多于ALI组同时间点.伤后0.5 h I-κBα含量开始明显下降,4 h达最低值,6 h后开始回升;GH组大鼠肺组织I-κBα含量下降更明显.相关性分析显示,PASAD、肺组织TNF和IL6水平及肺泡隔中性粒细胞浸润程度与NF-κB的表达、活化程度呈正相关.结论 NF-κB表达、活化在LPS诱导ALI的发病过程中有重要作用.应激反应阶段应用GH可加剧LPS诱导的ALI,其机制与促进肺局部NF-κB表达与活化而加剧肺局部炎症反应有关.
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