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Expression of heat shock protein 70 in intestinal mucosa during the early stage after severe burns and its significance
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1003-0603.2005.05.003
- Key Word:
- 热休克蛋白;肠黏膜;烧伤
Abstract： 目的探讨大鼠烧伤后早期肠黏膜组织热休克蛋白70(HSP70)的表达变化规律及其意义.方法采用大鼠烫伤模型,通过逆转录聚合酶链反应(RTPCR)、蛋白质免疫印迹(Western blot)及免疫组化等方法,检测伤后3、6、12、24和48 h不同时间点肠黏膜组织内HSP70及热休克因子1(HSF1)的表达分布情况.结果烫伤后3 h肠黏膜组织内HSP70 mRNA及蛋白表达均显著增加,分别在伤后6 h和12 h达高峰,伤后48 h仍高于正常对照组(P均<0.01);伤后3 h大鼠肠黏膜组织HSF1出现一过性降低,伤后6 h其表达显著高于正常对照组,并呈逐渐增加的趋势直至持续到伤后48 h(P均<0.01).结论严重烧伤早期肠黏膜组织HSP70及HSF1表达均显著增加,提示严重烧伤早期即可引起肠黏膜组织细胞的应激反应,可能与细胞的自我保护机制启动有关.
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