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Expression of thymosin β4 mRNA expression in keloid tissues and fibroblasts cultured from keloid and its significance
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1003-0603.2005.02.005
- Key Word:
- 胸腺素β4;瘢痕疙瘩;增生性瘢痕;半定量逆转录-聚合酶链反应
Abstract： 目的观察病理性瘢痕中胸腺素β4(TMSB4)mRNA的表达水平,探讨其表达水平与病理性瘢痕形成的关系.方法以组织块培养法于体外培养原代成纤维细胞.采用半定量逆转录聚合酶链反应(RT-PCR)技术,比较瘢痕疙瘩、增生性瘢痕和正常皮肤组织及其培养的3组成纤维细胞中TMSB4 mRNA表达水平的变化.结果在上述3种组织中,TMSB4 mRNA在瘢痕疙瘩中的表达量显著少于在增生性瘢痕和正常皮肤中的表达(P均<0.01),瘢痕疙瘩TMSB4 mRNA表达的平均值比增生性瘢痕减少66.98%,比正常皮肤减少62.48%.在上述3种组织培养的成纤维细胞中,TMSB4 mRNA在瘢痕疙瘩成纤维细胞中的表达量显著少于增生性瘢痕,平均减少27.13%(P<0.01);比在正常皮肤成纤维细胞中的表达平均减少16.07%,但差异无显著性.结论 TMSB4 mRNA在瘢痕疙瘩组织及其培养的成纤维细胞中的表达说明其与瘢痕疙瘩形成密切相关;TMSB4 mRNA在瘢痕疙瘩中表达减少可能是瘢痕疙瘩发病的重要致病因素之一.
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