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Analysis of the early and late gene expression of lipopolysaccharide activated macrophages by cDNA microarray
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1003-0603.2004.06.005
- Key Word:
- 内毒素;巨噬细胞;cDNA芯片;基因表达
Abstract： 目的利用cDNA芯片技术分析内毒素活化的小鼠腹腔巨噬细胞早期和晚期基因表达,以更全面了解内毒素在感染、创伤反应中通过巨噬细胞介导的炎症和免疫反应.方法以未刺激的和用1 mg/ L脂多糖(LPS)分别刺激2 h(早期)和24 h(晚期)的小鼠腹腔巨噬细胞制备33P标记的cDNA探针,并分别与小鼠cDNA表达芯片(含1 176个已知基因)杂交.结果巨噬细胞活化早期的3倍差异表达基因为69个,其中44个上调,25个下调;巨噬细胞活化晚期的3倍差异表达基因中有11个上调,26个下调;只有8个基因同时出现于活化早期和晚期的差异表达基因中.许多转录因子、细胞内信号转导调节蛋白、炎症细胞因子和细胞凋亡相关基因的表达均发生了明显的调节变化.发现BTB和CNC同源1(BACH1)、早期生长反应蛋白2(EGR2)、E47反应蛋白1(EIP1)、Ngfi-A结合蛋白2(NAB2)、成髓细胞白血病癌基因样蛋白(MYBL2)、神经纤维癌蛋白基因1(NF1)、睫状神经营养因子(CNTF)和Sema4A等一些以前未曾报道与LPS诱导的巨噬细胞活化相关的基因.结论采用cDNA芯片技术了解内毒素诱导的巨噬细胞活化早期和晚期的综合基因表达信息,有助于更好地了解感染、创伤后细菌内毒素诱导的炎症免疫反应.
- [1]Rong-Qian Wu,Ying-Xin Xu,Xu-Hua Song,etc.Relationship between cytokine mRNA expression and organ damage following cecal ligation and puncture[J].2002,(1).
- [2]盛志勇.努力提高脓毒症的认识水平[J].2003,(3).
- [3]D R, Wagner,C, McTiernan,V J, Sanders,etc.Adenosine inhibits lipopolysaccharide-induced secretion of tumor necrosis factor-alpha in the failing human heart.[J].1998,97(6).
- [4]Gold MR,Hancock RE,Finlay BB,etc.Salmonella typhimurium infection and lipopolysaccharide stimulation induce similar changes in macrophage gene expression.[J].2000,164(11).
- [5]Yi-Qing, Chen,Yong-Qing, Zhou,Ming-Hai, Wang.Activation of the RON receptor tyrosine kinase protects murine macrophages from apoptotic death induced by bacterial lipopolysaccharide.[J].2002,71.
- [6]Ludwick C,Staab A,Cabral GA,etc.Differential expression of the CB2 cannabinoid receptor by rodent macrophages and macrophage-like cells in relation to cell activation.[J].2002,2(1).
- [7]Lewis C.Sema7A is a Potent Monocyte Stimulator.[J].2002,56(3).
- [8]Georganas C,Walsh K,Pope RM,etc.FLICE-inhibitory protein expression during macrophage differentiation confers resistance to fas-mediated apoptosis.[J].1999,190(11).
- [9]Aderem A,Stacey KJ,Costelloe EO,etc.Mechanisms of regulation of the MacMARCKS gene in macrophages by bacterial lipopolysaccharide.[J].1999,66(3).
- [10]Scheerlinck JY,Jones KL,Phillips DJ,etc.Evidence for activin A and follistatin involvement in the systemic inflammatory response.[J].2001,180(1).