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Effects of fibroblast growth factor-10 on the secretions of transforming growth factor-α,plateletderived growth factor-AB and vascular endothelial grouth factor by normal adult human keratinocytes in culture
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1003-0603.2004.01.005
- Key Word:
- 成纤维细胞生长因子-10;血小板源性生长因子;转化生长因子;血管内皮细胞生长因子
Abstract： 目的观察成纤维细胞生长因子(FGF-10)对人角朊细胞表达转化生长因子-α(TGF-α)、血小板源性生长因子-AB(PDGF-AB)和血管内皮细胞生长因子(VEGF)的作用.方法 FGF-10工作浓度为4、16、125和500 μg/L,不含EGF和含EGF的无血清角朊细胞培养液分别作为阴性和阳性对照.细胞接种密度为2 500和375 000细胞/cm2,24、48和72 h进行培养上清中的细胞计数,TGF-α、PDGF-AB和VEGF的含量用酶联免疫吸附法(ELISA)测定.结果①低接种密度、细胞处于未融合生长状态时,各组24 h培养上清中TGF-α含量均较低,48 h的16 μg/L以上组及72 h各浓度组培养上清中TGF-α的分泌量均显著高于阴性对照组单个细胞的TGF-α分泌量.高接种密度、细胞处于融合生长状态时,24 h的培养上清中未检测到PDGF-AB;48和72 h的培养上清中,125和500 μg/L的FGF-10组PDGF-AB浓度及每106细胞的分泌量显著高于阴性对照组,且PDGF-AB的分泌呈FGF-10剂量依赖性.②低接种密度时,FGF-10各组VEGF浓度及每106细胞VEGF分泌量在各时间点均与阴性对照组无明显差异,而阳性对照EGF组VEGF分泌显著高于其它各组.结论 FGF-10上调角朊细胞分泌PDGF-AB、TNF-α可能与FGF-10间接作用于成纤维细胞、血管内皮细胞进而促进肉芽组织的形成有关.
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