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Effects of acute hypoxia and intermittent hypoxic acclimatization on erythropoietin and hypoxia-inducible factor-1α gene expression in rat hepatic and renal tissues
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1003-0603.2004.01.004
- Key Word:
- 低氧,急性;间断低氧习服;红细胞生成素;低氧诱导因子-1α
Abstract： 目的观察急性低氧和间断低氧习服对大鼠肝、肾组织内红细胞生成素(EPO)及其调控蛋白低氧诱导因子-1α(HIF-1α)基因表达的影响.方法用低压舱模拟高原低氧环境,进行3 000 m 2周、5 000 m 2周低氧训练,使大鼠达到低氧习服状态后,采用Northern斑点杂交研究间断低氧习服前后,8 000 m 4 h低氧处理的大鼠肝、肾组织内EPO和HIF-1α基因表达的变化.结果与常氧对照组相比,急性低氧处理的大鼠肝、肾组织内EPO基因表达都明显增强(P<0.05或P<0.01);而间断低氧习服后,同样的低氧处理的大鼠肝、肾组织中EPO基因表达都明显低于急性低氧组(P<0.01),EPO mRNA含量与常氧对照组水平差异不显著(P>0.05).急性低氧处理的大鼠肝组织中HIF-1α基因表达水平明显高于常氧对照组和间断低氧习服组(P均<0.01),而在不同处理的肾组织中,该基因表达水平差异不明显(P均>0.05).结论急性低氧能够刺激肝、肾组织内EPO基因表达;而间断低氧习服的大鼠耐低氧能力明显增强,肝、肾组织内EPO基因表达水平可恢复到常氧对照组水平.HIF-1α在低氧影响EPO基因表达的过程中可能起着重要的调节作用.
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