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Study on the relationship of heat shock protein 70 with secondary liver damage in early stage after severe multiple injury

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Author:: No author available

Journal Title:: CHINESE CRITICAL CARE MEDICINE

Issue:: 4

DOI:: 10.3760/j.issn:1003-0603.2003.04.004

Key Word:: 创伤;热休克蛋白类;肝脏继发性损害

Abstract：目的:探讨热休克蛋白70(HSP70)在严重多发伤后肝脏继发性损害中的作用.方法:成年雄性Wistar大鼠,采用严重胸部撞击伤伴单侧股骨骨折多发伤模型.动态观察致伤后24 h及用糖皮质激素受体阻断剂米非司酮后大鼠肝组织HSP70、肝功能指标及致伤24 h死亡率等变化.HSP70表达测定采用免疫印迹法,并进行计算机图像分析.结果:伤后HSP70在肝组织中表达伤后迅速增加,8 h达到峰值,24 h仍维持在较高水平;但血清丙氨酸转氨酶(ALT)、总胆红素(TB)及白蛋白的改变无显著差异.使用糖皮质激素受体阻断剂米非司酮能使肝组织HSP70表达明显增多;血清ALT及TB在伤后早期即有明显升高(P均<0.01),白蛋白明显下降(P<0.01);伤后24 h动物死亡率明显增加(P<0.01).结论:HSP70可能参与了肝组织细胞抗损伤机制的启动,但HSP70过高表达则可能对肝脏造成损害.HSP70可作为创伤后肝损伤与抗损伤机制的一个重要指标.

Reference

[1]袁志强,李晓鲁,彭毅志.热休克预处理对肠上皮细胞缺氧-再给氧损伤的保护作用及其机制[J].2002,(5).
[2]任成山,陆海华,杜晓锋,等.重度创伤后全身炎症反应综合征和多器官功能障碍综合征[J].2001,(11).
[3]王正国,孙立英,刘宝松.生物撞击机的研制及撞击伤发生机制的研究[J].1999,(4).
[4]J, Fan,X Q, Gong,J, Wu,etc.Effect of glucocorticoid receptor (GR) blockade on endotoxemia in rats.[J].1994,42(2).
[5]Aya Fukuda,Toshihiko Osawa,Hiroaki Oda,etc.Oxidative Stress Response in Iron-Induced Acute Nephrotoxicity: Enhanced Expression of Heat Shock Protein 90[J].1996,219(1).
[6]A M, Planas,M A, Soriano,A, Estrada,etc.The heat shock stress response after brain lesions: induction of 72 kDa heat shock protein (cell types involved, axonal transport, transcriptional regulation) and protein synthesis inhibition.[J].1997,51(6).