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Expression of protooncogene cjun,p38 and proliferating cell nuclear antigen in intestinal epithelial cells in rats different development stages
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1003-0603.2003.02.004
- Key Word:
- 原癌基因c-jun;p38;小肠上皮细胞;增殖分化;大鼠
Abstract： 目的:研究原癌基因 c-jun及其相关基因p38在不同发育阶段大鼠小肠上皮细胞的表达分布特征,探讨c-jun和p38基因在小肠创伤修复时可能发挥的生理学作用.方法:利用超敏的链霉卵白素(SP)免疫组织化学方法,观察c-jun和p38基因在胚胎、新生及成年各时间段Wistar大鼠小肠上皮细胞的阳性表达与分布特征.同时以细胞增殖活性的标志增殖细胞核抗原(PCNA)表达为参照,对不同时期细胞的增殖分化状况作对比研究.结果:在胚胎第17 d(E17 d)、E19 d及新生期第1 d(P1 d)、P2 d、P7 d、P14 d和P28 d,小肠上皮细胞c-jun基因的阳性表达范围广泛,并且随着肠绒毛细胞的发育成熟,其分布从绒毛及间隙转移至绒毛上部细胞;在此期间,PCNA阳性细胞主要分布于新生绒毛底部、绒毛间隙、隐窝内的细胞;到成年期大鼠,c-jun基因的表达主要在小肠绒毛顶部,而PCNA阳性细胞也只局限在小肠隐窝.从E17 d至成年期,p38的阳性表达主要集中在绒毛间隙和陷窝内核分裂细胞.结论:在小肠发育的早期,原癌基因c-jun在特定位置的高度表达,可能与细胞的快速分化有关;而其相关基因p38的表达可能与细胞有丝分裂存在着一定的内在联系.
- [1]邢峰,付小兵,杨银辉,等.小肠缺血-再灌注后磷酸化p38丝裂原活化蛋白激酶的表达特征及与干细胞定位的关系[J].2002,(9).
- [2]Xu R.-H..AP-1\/Jun Is Required for Early Xenopus Development and Mediates Mesoderm Induction by Fibroblast Growth Factor but Not by Activin[J].1996.
- [3]Iwata, A.,Masago, A.,Yamada, K..Expression of Basic Fibroblast Growth Factor mRNA after Transient Focal Ischemia: Comparison with Expression of c-fos, c-jun, and hsp 70 mRNA[J].1997,14(4).
- [4]B, Binétruy,T, Smeal,M, Karin.Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain.[J].1991,351(6322).
- [5]Kung HF,Lin JJ,Kim J,etc.Mesoderm induction by heterodimeric AP-1 (c-Jun and c-Fos) and its involvement in mesoderm formation through the embryonic fibroblast growth factor/Xbra autocatalytic loop during the early development of Xenopus embryos.[J].1998,273(3).
- [6]Hua Zheng,Jie Liu,K.H. Andy Choo,etc.Metallothionein-I and -II Knock-Out Mice Are Sensitive to Cadmium-Induced Liver mRNA Expression of c-junandp53[J].1996,136(2).
- [7]付小兵.现代创伤修复学[M].2002.