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Characteristics of proliferating cell nuclear antigen and P53 expression in fetal and postnatal skins and their biological significance
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 11
- DOI:
- 10.3760/j.issn:1003-0603.2002.11.004
- Key Word:
- 增殖细胞核抗原;P53蛋白;皮肤组织;胎儿
Abstract： 目的:探讨增殖细胞核抗原(P CNA)和P53蛋白在胎儿与成人皮肤组织中的表达特征及其可能的生物学意义.方法:用免疫组织化学方法和病理技术确定这两种蛋白在不同胎龄的胎儿皮肤(8例)和成人皮肤(8例)中的定位和表达量.结果:在妊娠早期和中期胎儿的皮肤组织中,PCNA呈强阳性表达,而P53蛋白表达很低.随着胎儿生长发育,PCNA蛋白的阳性细胞率逐渐降低,而P53蛋白表达逐渐增强. 在发育后期的胎儿和成人皮肤中,PCNA的阳性细胞率显著下降,阳性信号主要分布于角质形成细胞和毛囊上皮细胞的胞核内;P53呈阳性表达,蛋白颗粒定位于表皮基底层细胞和部分成纤维细胞中.结论:在不同发育阶段皮肤组织中,PCNA和P53蛋白可能通过影响细胞内DN A合成和修复,调节皮肤组织的生长发育、结构和功能的维持以及损伤后的修复.
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