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Effect of epidermal growth factor on gut function a nd mucosal permeability in rats after gut ischemia-reperfusion injury
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 11
- DOI:
- 10.3760/j.issn:1003-0603.2002.11.003
- Key Word:
- 表皮生长因子;缺血再灌注损伤,肠道;肠黏膜通透 性;大鼠
Abstract： 目的:观察大鼠肠缺血再灌注后外源性表皮生长因子(EGF)对肠黏膜通透性和肠、肝、肾功能改变的影响 .方法:80只3级雄性Wistar大鼠,随机分为假手术(C)组、肠缺血(I)组、肠缺血再灌注(IR)组和EGF治疗组.IR组和EGF治疗组均用微血管夹夹闭肠系膜上动脉根部45分钟,之后放松血管夹形成再灌注.在再灌注即刻经颈静脉分别注入EGF 100 μg/kg或生理盐水,分别于伤后2、6、12和24小时将动物活杀.C组仅分离肠系膜上动脉根部而不夹闭,I组在缺血45分钟后即刻活杀.取血检测肝、肾功能, 血浆二胺氧化酶(DAO)活性和D乳酸浓度;取小肠、肝和肾组织进行形态学观察 .结果:①与C组相比,各组动物血浆丙氨酸转氨酶(ALT) 和尿素氮(BUN)均明显升高,但EGF治疗组升高的幅度显著低于IR组(P <0.05).②EGF治疗组的血浆D乳酸浓度和DAO活性在伤后升高的幅度均明显低于IR组(P<0.01).③EGF治疗组肝、肾和小肠黏膜充血、水肿、炎细胞浸润及局灶性坏死的程度较IR组显著减轻 .结论:伤后给予外源性EGF显著减轻了缺血再灌注所致肠、肝、肾功能的损伤,其主要作用环节是促进了EGF与受体的结合及随之发生的信号传导.
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