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Expression characteristics of phosphorylating-p38 mitogen-activated protein kinase in rat small intestine after ischemia-reperfusion injury and its relationship with the localization of intestinal stem cells
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 9
- DOI:
- 10.3760/j.issn:1003-0603.2002.09.003
- Key Word:
- p38丝裂原活化蛋白激酶;干细胞;肠道;缺血-再灌注损伤;大鼠
Abstract： 目的:观察大鼠肠缺血-再灌注(I-R)后磷酸化p38丝裂原活化蛋白激酶(MAPK)的表达特征及与干细胞定位的关系.方法:雄性Wistar大鼠48只,随机分为假手术组(C)、肠缺血组(I)和肠缺血-再灌注组(R).C组仅分离肠系膜上动脉根部而不夹闭;I组用微血管夹夹闭肠系膜上动脉根部45分钟后即刻活杀; R组动物在缺血45分钟之后放松血管夹,分别于再灌注后2、6、12和24小时活杀.取血检测血浆二胺氧化酶(DAO)活性,取小肠组织进行形态学观察,用免疫组织化学方法研究磷酸化p38的表达特征.结果:与C组相比,I组和R组血浆DAO活性升高,R组动物的小肠黏膜表现为充血、水肿、炎细胞浸润及坏死糜烂,以再灌注后12小时最显著.磷酸化p38染色显示,C组和I组大鼠每个小肠隐窝仅有5～6个阳性细胞,主要在隐窝的下1/2,大部分表现为分布在胞浆中的棕色颗粒,与肠道干细胞及其初级子代细胞的位置一致.再灌注后2小时阳性细胞数量下降,在6小时隐窝底部的阳性细胞数量增多,主要分布在隐窝的中下1/3,在12小时达到高峰,为隐窝细胞总数的35.6%,在24小时明显下降接近正常.绒毛基质偶有阳性细胞,绒毛上皮无表达.结论:磷酸化p38在肠道的表达与干细胞及其初级子代细胞的分布非常接近,肠I-R使其表达增加,表明磷酸化p38与肠道干细胞之间存在密切的关系,有可能作为干细胞的标记物.
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