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Effects of antisense RNA of sodium hydrogen exchanger-1 on proliferation of pulmonary artery smooth muscle cells of rats in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 9
- DOI:
- 10.3760/j.issn:1003-0603.2002.09.002
- Key Word:
- 反义RNA;Na+/H+交换器;肺动脉平滑肌细胞;增殖
Abstract： 目的:探讨反义RNA对大鼠肺动脉平滑肌细胞Na+/H+交换器(NHE)-1表达和活性、细胞内pH(pHi)的影响,及其与细胞增殖的关系.方法:构建含NHE-1反义RNA序列的pLXSN反转录病毒重组载体,将其转染体外培养的大鼠肺动脉平滑肌细胞,G418筛选后获取含有重组载体的细胞克隆,检测细胞NHE-1 mRNA表达、22Na摄取量、pHi和3H-TdR掺入量.结果:与转染pLXSN空载体的细胞和正常对照组细胞比较,转染了重组载体的肺动脉平滑肌细胞中NHE-1 mRNA表达、22Na摄取量明显减少,同时伴有pHi降低和3H-TdR掺入量减少,正常对照组和空载体组间无显著差异.结论:NHE-1反义RNA可以减少NHE-1表达,从而诱导细胞酸化,抑制肺动脉平滑肌细胞增殖.
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