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Effect of recombinant bact ericidal-permeability increasing protein on hepatic expression of pro -inflammatory cytokine and ant i-inflammatory cytokine in septic rats
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1003-0603.2002.05.004
- Key Word:
- 脓毒症;盲肠结扎穿孔术;杀菌-通透性增加蛋白;细胞因子,致炎,抗炎
Abstract： 目的:探讨杀菌- 通透性增加蛋白(BPI)对脓毒症大鼠肝组织致炎与抗炎细胞因子表达的影响.方法:采用大鼠盲肠结扎穿孔(CLP)致脓毒症模型,动物随机分为正常对照组、脓毒症组和BPI治疗组.分别于CLP后12和24小时处死动物,检测肝组织肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)mRN A及其蛋白表达以及肝功能指标的改变.结果:脓毒症动物肝组织TNF-α及IL-10基因和蛋白表达均显著升高(P<0.05或P<0.01),其中CLP后12小时组织TNF α蛋白水平升高幅度明显大于IL-10的改变.BPI治疗12小时组TNFα基因及蛋白均显著下降(P<0.05或P<0.01),而IL-1 0则不同程度地升高;同时,治疗组12小时肝功能指标亦明显改善.结论:脓毒症早期应用BPI治疗可明显抑制肝组织TNF-α等致炎细胞因子表达,并上调局部组织IL-10等抗炎细胞因子的产生,从而有助于恢复体内炎症反应平衡与减轻脓毒症所致肝损伤.
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