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Protective effect of heat shock pretreatment on hypoxia-r eoxygenation induced injury of intestinal epithelial cells in vitro and its mec hanism
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1003-0603.2002.05.003
- Key Word:
- 热休克蛋白;凋亡;缺氧-再给氧;肠上皮细胞;热休克预处理
Abstract： 目的:观察热休克预处理对肠上皮细胞(IEC-6)缺氧-再给氧损伤的影响,并探讨热休克蛋白70 (HSP70)的细胞保护作用.方法:体外培养IEC-6细胞,分为正常对照组、单纯缺氧-再给氧组及热休克预处理组,观察缺氧-再给氧后各组细胞HSP70及Bcl -2表达及细胞活力、乳酸脱氢酶(LDH)漏出情况;用流式细胞仪检测细胞凋亡情况.结果:热休克预处理可诱导HSP70产生(P<0.01),显著增加缺氧 -再给氧处理后IEC-6细胞活力(P<0.05),使LDH漏出减少(P<0.05),凋亡相关基因Bcl-2表达明显增加(P<0.01),细胞凋亡率明显降低(P<0.01).结论:热休克预处理可能通过诱导HSP70表达来减轻肠上皮细胞缺氧-再给氧损伤;增加细胞Bcl-2基因表达并抑制肠上皮细胞缺氧-给氧后细胞凋亡可能是其保护作用机制之一.
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