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Effect of recombinant adenovirusmediated brain derived neurotrophic factor gene transfer on induced nitric oxide synthase expression and apoptosis after traumatic brain injury in rats
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1003-0603.2002.01.001
- Key Word:
- 重组腺病毒;脑源性神经营养因子;一氧化氮合酶;脑损伤,创伤性;细胞凋亡
Abstract： 目的:研究重组腺病毒介导的脑源性神经营养因子(BDNF)基因转移对创伤性脑损伤(TBI)后诱导型一氧化氮合酶(iNOS)表达及细胞凋亡的影响.方法:将4 μl重组腺病毒载体注入承受单侧大脑皮质重锤打击大鼠的海马,对照组注射病毒缓冲液.伤后3小时及1、3、7和14日,利用免疫组织化学单标和双标染色、原位杂交/组织化学染色及DNA原位末端标记等方法,检测大鼠伤侧大脑皮质和海马各区iNOS、BDNF及凋亡相关信号表达的改变.结果:与对照组相比,各损伤组大鼠大脑皮质及海马各区iNOS阳性细胞于伤后3小时开始显著增多,3日达高峰.多数iNOS阳性细胞同时呈现凋亡相关蛋白阳性反应或分子生物学标记方法(TUNEL)阳性反应,但很少同时表达BDNF mRNA.注射病毒载体组伤后3日和7日,海马CA1区和海马齿状回门区(DH)表达iNOS、凋亡相关蛋白的细胞及凋亡细胞均显著减少(P均<0.01),而表达BDNF mRNA的神经元显著增多.结论:TBI诱导海马细胞表达iNOS及诱导海马细胞凋亡;腺病毒介导的BDNF基因转移通过下调iNOS表达,增加BDNF表达及减少细胞凋亡而保护海马神经元.
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