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Changes in 1,4,5-trisphosphate receptor expression in pulmonary arter y smooth muscle cells of pulmonary hypertensive rats
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 12
- DOI:
- 10.3760/j.issn:1003-0603.2001.12.005
- Key Word:
- 1,4,5-三磷酸肌醇受体;野百合碱;肺动脉高压;血管平滑肌;钙
Abstract： 目的:检测肺动脉高压(PAH)大鼠肺动脉平滑肌细胞(PASMCs)上1,4,5-三磷酸肌醇受体(IP3R)的亚型及IP3R各亚型在PAH时表达水平的变化.方法:采用大鼠一次性腹腔注射野百合碱(MCT)60 mg/kg复制PAH动物模型,用Western blot法检测IP3R亚型的表达及在PAH状态下其蛋白水平表达的变化.结果:大鼠PASMCs共同表达3个IP3R亚型,在PAH时IP3R1表达水平明显增高,约为对照组的2倍(P<0.01),而IP3R2和IP3R3的表达水平无明显变化.结论:PASMCs的IP3R1表达增强可能参与了PAH的发生.
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