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Comparison of Schwann cell culture methods in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 9
- DOI:
- 10.3760/j.issn:1003-0603.2001.09.006
- Key Word:
- 雪旺氏细胞;植块;刺激因子
Abstract： 目的:寻找一个快捷、简便、获取大量雪旺氏(Schwann)细胞的培养方法,旨在为临床Schwann细胞的自体移植提供实验基础.方法:比较了4种Schwann细胞培养方法,即植块反复种植法、单细胞悬液法、阿糖胞苷抑制成纤维细胞生长法和刺激因子增殖法.结果:植块反复种植法加刺激因子forskolin和BTI可使Schwann细胞的产量明显高于其它组,Schwann细胞纯度可达98%,且成纤维细胞明显减少.结论:植块反复种植法加刺激因子forskolin和BTI为Schwann细胞培养的最佳方法.此方法简便、快速,可在短时间内获得大量Schwann细胞.
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