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The significance of changes in mGluR5 mRNA in brain cortex after infrasonic damage in rats
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 8
- DOI:
- 10.3760/j.issn:1003-0603.2001.08.006
- Key Word:
- 脑损伤,次声;代谢性谷氨酸受体5亚型
Abstract： 目的:探讨8 Hz、120 dB次声作用后大鼠脑皮质内代谢性谷氨酸受体5亚型(mGluR5) mRNA表达的变化及意义.方法:SD大鼠60只被随机分为对照组及次声作用1、7、14次组和次声作用组,采用自制的次声压力舱用8 Hz、120 dB的次声按规定次数,每次作用 2小时.利用地高辛标记寡聚核苷酸探针原位杂交技术与计算机图像分析系统,在光镜与透射电镜下观察mGluR5分别在额、顶、颞及枕皮质1区阳性神经元表达的改变,以及相应皮质的病理变化.结果:次声作用1次组,额、顶、颞、枕脑皮质内mGluR5 mRNA阳性神经元表达减少,次声作用7次组脑皮质内mGluR5 mRNA阳性神经元表达数减少至谷底与对照组和次声作用1次组比较P均<0.01,次声作用14次组脑皮质内mGluR5 mRNA阳性神经元表达数基本恢复到正常水平;阳性神经元在脑皮质浅层散在分布,mGluR5 mRNA在胞浆和胞核内均匀地呈颗粒状表达.结论:次声脑损伤可抑制mGluR5的表达和生成,其作用可能与mGluR1α不同,参与机体对次声脑损伤的保护.
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