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The effect of intraventricular administration of insulin on expression of Bcl-xL protein and apoptosis of neuron in rat hippocampal CA1 region
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 8
- DOI:
- 10.3760/j.issn:1003-0603.2001.08.005
- Key Word:
- 胰岛素;脑缺血;Bcl基因;细胞凋亡
Abstract： 目的:探讨胰岛素对全脑缺血后海马CA1区Bcl-xL蛋白表达及神经元凋亡的影响.方法:利用四血管闭塞法制作全脑缺血大鼠模型.造成脑缺血15分钟后行再灌注 ,于再灌注后即刻经脑室注入1 U胰岛素,利用免疫组织化学及原位标记法分别于全脑缺血后1、3日观察海马CA1区Bcl-xL蛋白表达及神经元凋亡的情况.结果:全脑缺血后1日,缺血组及胰岛素治疗组鼠海马CA1区未见原位标记阳性细胞.治疗组鼠海马CA1区可见呈阳性表达的Bcl-xL蛋白,而缺血组鼠海马CA1区Bcl-xL蛋白的表达则呈阴性.全脑缺血后3日,缺血组鼠海马CA1区Bcl-xL蛋白的表达仍呈阴性,海马CA1区原位标记阳性细胞计数为(143.5±11.6)个.治疗组鼠海马CA1区Bcl-xL蛋白呈阳性表达,海马CA1区原位标记阳性细胞计数为(75.6±6.7)个.结论:全脑缺血后脑室内注入胰岛素具有促进海马CA1区Bcl-xL蛋白表达及减少神经元凋亡的中枢直接保护作用.
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