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The expression of brain derived neurotrophic factor mRNA in rats after fluid percussion brain injury
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 8
- DOI:
- 10.3760/j.issn:1003-0603.2001.08.004
- Key Word:
- 液压冲击脑损伤;脑源性神经营养因子;免疫组织化学;原位杂交;图像分析;大鼠
Abstract： 目的:研究脑损伤后脑源性神经营养因子(BDNF)mRNA表达及其时序性变化,进一步研究脑损伤的分子机制.方法:用免疫组织化学及原位杂交方法,观察液压冲击脑损伤大鼠海马BDNF mRNA受伤后不同时间(5、15、30分钟和1、3、6、12小时及1、3、7日) 的表达情况,以正常大鼠及手术大鼠作为对照.结果:正常及手术对照组大鼠脑组织海马表达少量的BDNF;脑损伤后1小时海马齿状回CA2及CA3细胞表达增强,3～6小时达高峰,12小时开始下降,24小时后降至对照水平.1、3、6和12小时与正常及手术对照组比较均有显著性差异.结论:液压冲击脑损伤可诱导BDNF基因的表达,BDNF mRNA表达存在时间差异,提示机体对脑损伤存在自身保护机制.
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