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The expression of Bcl-2,Bcl-xL and Bax protein in rat hippocampal CA1 region following the global brain ischemia
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 8
- DOI:
- 10.3760/j.issn:1003-0603.2001.08.003
- Key Word:
- 基因,Bcl-2,Bcl-xL,Bax;选择性敏感;缺血再灌注,全脑,大鼠
Abstract： 目的:通过系统观察大鼠全脑缺血再灌注后不同时相海马CA1区神经元Bcl-2、Bcl-xL及Bax蛋白表达的改变,探讨该区神经元短暂脑缺血后产生选择性敏感的机制.方法:通过四血管闭塞法建立大鼠全脑缺血再灌注模型,利用免疫组织化学方法观察再灌注后1、3、5日海马CA1区神经元Bcl-2、Bcl-xL及Bax蛋白表达的情况;并同时与海马CA1区神经元的病理形态学改变进行对比.结果:全脑缺血再灌注后1日,海马CA1区Bcl-2和Bcl-xL蛋白呈阴性表达,Bax蛋白呈弱阳性表达;海马CA1区神经元未见明显形态学改变.全脑缺血再灌注后3日,海马CA1区 Bcl-2和Bcl-xL蛋白表达仍为阴性,而Bax蛋白则呈明显阳性表达;海马CA1区可见部分神经元死亡;全脑缺血再灌注后5日,海马CA1区 Bcl-2、Bcl-xL及Bax蛋白则呈阴性表达;海马CA1区神经元大部分死亡.结论:全脑缺血再灌注后海马CA1区神经元Bcl-2和Bcl-xL蛋白表达的抑制可能是其对短暂脑缺血产生选择性敏感的机制之一.
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