You Position: Home > Paper
The expression of nuclear factorkappa B in mice spleen lymphocytes after trauma
( views:55, downloads:8 )
- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 7
- DOI:
- 10.3760/j.issn:1003-0603.2001.07.003
- Key Word:
- 创伤;核因子κB;脾细胞;核转录因子;全身炎症反应综合征
Abstract： 目的：探讨创伤后不同时间点脾细胞核因子kappa B(NFκB)的DNA结合蛋白和NFκB p65蛋白亚型表达的动态变化。方法：采用小鼠双后肢闭合性砸伤+骨折模型，于创伤后1、4、7日处死动物，分离、纯化脾细胞，提取脾细胞核蛋白。用电泳迁移率改变试验(EMSA）检测NFκB的DNA结合活性；用免疫蛋白印迹法（Western blot）检测NFκB p65蛋白亚型表达。结果：脾细胞NFκB的DNA结合活性在创伤后1、4、7日表达明显增高。NFκB p65蛋白亚型在创伤后1、4、7日表达明显增加。结论：创伤可明显增强脾细胞NFκB的DNA结合活性和p65蛋白亚型的表达。在创伤后淋巴细胞活化及全身炎症反应综合征(SIRS)发生、发展过程中可能起重要作用。
- [1]M A, Pahlavani,M D, Harris,A, Richardson.The increase in the induction of IL-2 expression with caloric restriction is correlated to changes in the transcription factor NFAT.[J].1997,180(1).
- [2]T S, Samy,A, Ayala,R A, Catania,etc.Trauma-hemorrhage activates signal transduction pathways in mouse splenic T cells.[J].1998,9(6).
- [3]梁华平,王正国,耿波,等.创伤后巨噬细胞对T细胞白介素2及白介素2受体α基因表达的直接接触抑制作用[J].1995,(2).
- [4]J D, Dignam,R M, Lebovitz,R G, Roeder.Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.[J].1983,11(5).
- [5]梁华平,张艳,耿波.创伤小鼠白细胞介素-6的变化[J].1994,(3).
- [6]梁华平,付培蓉,耿波,等.创伤对小鼠淋巴细胞激活作用的研究[J].1993,(1).
- [7]S, Ghosh,M J, May,E B, Kopp.NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses.[J].1998,16.
- [8]Rodrick ML,Mannick JA,Lederer JA.The effects of injury on the adaptive immune response.[J].1999,11(3).
- [9]Mondino A,Whaley CD,DeSilva DR,etc.Defective transcription of the IL-2 gene is associated with impaired expression of c-Fos, FosB, and JunB in anergic T helper 1 cells.[J].1996,157(5).
- [10]Samy TS,Chung CS,Bland KI,etc.Proteasome participates in the alteration of signal transduction in T and B lymphocytes following trauma-hemorrhage.[J].1999,1453(1).
- [11]Kelly JL,Lederer JA,Lyons A,etc.Burn injury primes naive CD4+ T cells for an augmented T-helper 1 response.[J].1998,124(2).