Experimental model of acute respiratory distress syndrome in sheep induced by a low dose of lipopolysaccharide
- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1003-0603.2001.02.009
- Key Word:
- 呼吸窘迫综合征,急性;小剂量内毒素;动物模型,绵羊
Abstract： Objective:To establish a sheep model of acute respiratorydistress syndrome (ARDS) by intravenously infusing a low dose of lipo polysaccharide (LPS).Methods:In 13 anaesthetized sheep,36 μg/kg LPS were intravenously in fused within 3060 minutes.Hemodynamics,lung mechanics and gas exchan ge were observed every half an hour.Results:After (3.1±1.2) hours of LPS infusion,ARDS model was successfully reproduced in 10 sheep conforming to ARDS criteria.Oxygenation index significantly decreased after 1.0 hour of LPS infusion (P<0.05),and reached const an tvalues 3.0 hours later.Cardiac index significantly decreased after 1.0 houe level at 3.5 hours;so did oxygen delivery (P<0.05 ) and became constant 3.5 hours later.After 1.5 hours of LPS infusion,airway resistance,peak inspiratory pressure and mean airway pr essu re significantly increased (t=2.8,4.2,5.3,all P<0.05),but dynamic compliance markedly decreased (t=3.1,P<0.05), becoming constant gradually at 3.0 hours,2.5 hours respectively.Conclusions:It is practical and stable ARDS sheep model by intraveno usly infusing a low does of LPS.
- [1]张海涛,晁彦公,马遂,等.静脉注射内毒素建立麻醉状态下绵羊急性呼吸窘迫综合征模型[J].1999,(2).
- [2]张海涛,马遂,晁彦公.应用呼气末正压治疗早期急性呼吸窘迫综合征的实验研究[J].1998,(6).
- [3]K L, Brigham,B, Meyrick.Endotoxin and lung injury.[J].1986,133(5).
- [4]A M, Esbenshade,J H, Newman,P M, Lams,etc.Respiratory failure after endotoxin infusion in sheep: lung mechanics and lung fluid balance.[J].1982,53.
- [5]胡森,盛志勇,周宝桐.MODS动物模型研究进展[J].1999,(8).
- [6]K L, Brigham,R, Bowers,J, Haynes.Increased sheep lung vascular permeability caused by Escherichia coli endotoxin.[J].1979,45.
- [7]Bernard G R,Artigas A,Brigham K L,etc.The American-European Consensus Conference on ARDS. Definitions, mechanisms, relevant outcomes, and clinical trial coordination.[J].1994.
- [8]胡森.多器官功能障碍综合征动物模型的系列研究[J].1996,(1).
- [9]Dr.A.Sohma,W.J.Brampton,M.S.Dunnill,etc.Effect of ventilation with positive end-expiratory pressure on the development of lung damage in experimental acid aspiration pneumonia in the rabbit[J].1992,18(2).
- [10]Mang H,Kacmarek R M,Ritz R,etc.Cardiorespiratory effects of volume- and pressure-controlled ventilation at various I\/E ratios in an acute lung injury model.[J].1995.
- [11]R A, Mustard,J, Fisher,S, Hayman,etc.Cardiopulmonary responses to Pseudomonas septicemia in swine: an improved model of the adult respiratory distress syndrome.[J].1989,39(1).