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Intercellular adhesion molecule1 expression and neutrophil infiltration after cerebral ischemiareperfusion inrats
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1673-4904.2000.05.017
- Key Word:
- 缺血再灌注损伤,脑;细胞间粘附分子1;白细胞浸润
Abstract： 目的:观察大鼠脑缺血再灌注后不同时间缺血区细胞间粘附分子1(ICAM1)的表达和中性粒细胞的浸润以及神经细胞的损伤情况.方法:40只Wistar大鼠分为对照组、假手术组和缺血2小时再灌注2、4、12、24、48、96小时组,分别用免疫组织化学和组织切片方法检测大脑中动脉阻塞2小时再灌注不同时间点局部脑组织ICAM1蛋白表达及中性粒细胞浸润情况.结果:大鼠脑缺血再灌注2小时局部脑组织ICAM 1的表达开始升高,于再灌注48小时达到峰值,持续至再灌注96小时仍保持较高水平表达; 局部缺血区域中性粒细胞浸润于再灌注后12小时开始升高,48小时达到高峰,与ICAM1的表达呈同步改变.结论:脑缺血再灌注后表达增高的ICAM1与局部缺血区中性粒细胞的浸润密切相关,这一过程是导致缺血再灌注损伤的一个重要机制.
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