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The potential role of leukocyte in postasphyxial renal injury in neonatal rats and its pathogenic mechanism
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1674-6554.2000.04.001
- Key Word:
- 窒息;肾损伤;白细胞;细胞因子
Abstract： 目的:探讨白细胞在新生大白鼠窒息后肾损伤中的作用及机制.方法:将20只7～10日龄Wistar大白鼠随机分为窒息组和对照组,每组10只,制成常压窒息模型.在窒息30分钟复氧120分钟时,取左肾称重求出左肾系数(LRC),并分别采用硫代巴比妥酸法、酶联免疫吸附法(ELISA)检测肾组织过氧化脂质(LPO)、肿瘤坏死因子-α(TNF-α)和白介素8-(IL-8)的含量,同时在光镜下对肾小管损伤程度进行评分,并计数肾微血管床的白细胞滞留数.结果:窒息后复氧120分钟时,白细胞滞留数、左肾系数、肾小管评分及组织中LPO、TNF-α、IL-8的含量均较对照组显著升高(P均<0.05),且白细胞滞留数与左肾系数、肾小管评分、LPO、TNF-α和IL-8含量之间呈显著正相关关系(r分别为0.49、0.55、0.51、0.48、0.70,P均<0.05).结论:窒息后肾组织炎性细胞因子的产生和白细胞滞留相互作用是造成窒息后肾损伤的重要环节,对临床上采用抗炎或抗白细胞措施防治窒息后肾损伤有重要价值.
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