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Protective effects of endogenous basic fibroblast growth factor activated by 2,3 butanedion monoxime on functional changes of ischemic intestine, liver and kidney in rats
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1671-0290.2000.03.001
- Key Word:
- 损伤,缺血再灌注;肠道;肝;肾;成纤维细胞生长因子,碱性;2,3丁二酮
Abstract： 目的:研究激活内源性碱性成纤维细胞生长因子(bFGF)对肠系膜上动脉(SMA)夹闭所致肠、肝、肾损伤的保护作用及其可能的机制.方法:24只大鼠分成内源性bFGF激活A组、bFGF治疗B组、生理盐水对照治疗C组以及正常对照D组.A组于SMA夹闭前静脉推注2,3丁二酮类化合物(BDM)生理盐水0.15 ml(每只鼠含BDM 40 mg);B、C组分别于SMA夹闭45分钟后于松夹即刻从右颈外静脉分别注入0.15 ml bFGF肝素生理盐水(每只鼠bFGF 2 μg)和0.15 ml肝素生理盐水;D组仅分离SMA,但不夹闭.各组分别于缺血45分钟即刻及再灌注后6、24和48小时将动物活杀,取血标本和肠、肝、肾组织分别测定肝、肾功能与光镜观察组织形态学结构.结果:采用BDM不仅可以显著改善脏器病理形态学结构,而且可以显著减轻伤后6小时肝、肾功能损害,与生理盐水对照治疗组相比差异显著,其中2组动物血浆丙氨酸转氨酶和血尿素氮值分别为(696.8±108.4)nmol·s-1 ·L-1和(5.4±2.5)mmol/L比(1 812.0±640.1)nmol·s-1 ·L-1和(11.3±4.9)mmol/L,P均<0.05.同样采用bFGF治疗的动物也具有相似的防治效果.结论:激活内源性bFGF有助于减轻缺血再灌注所致的肠、肝以及肾等组织损害.
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