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The role of protease-antiprotease imbalance in acute lung injury induced by endotoxin in rabbits
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1003-0603.2000.01.003
- Key Word:
- 肺损伤,急性;α1-抗胰蛋白酶;弹性蛋白酶,中性粒细胞;兔;内毒素
Abstract： 目的:研究蛋白酶-抗蛋白酶失衡在内毒素诱发急性肺损伤(ALI)兔模型中的作用.方法:成年健康新西兰大白兔16只经麻醉、气管切开插管后给予机械通气.随机分成2组(每组8只):即内毒素(LPS)致ALI模型组(LPS组)和生理盐水对照组(NS组).监测动脉血氧分压(PaO2)、动脉血压(BP)、外周血白细胞数(WBC)、气道峰压(Ppeak)、静态总呼吸顺应性(TRC)和肺内动静脉分流(s/t)等指标的变化;比较实验8小时后血浆α1-抗胰蛋白酶(α1-AT)含量和活性、支气管肺泡灌洗液(BALF)中总蛋白(TP)含量、中性粒细胞弹性蛋白酶(NE)样活性、α1-AT活性、白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、总磷脂(TPL)、饱和磷脂酰胆碱(DSPC)含量及肺湿重与干重比(W/D)的差异.结果:与基础值相比,静注LPS后PaO2下降,氧合指数<26.67 kPa(1 kPa=7.5 mmHg),TRC下降>50%,肺内动静脉分流增加>25%;与NS组相比,血浆α1-AT含量增高但活性下降,外周血白细胞减少,而BALF中TP含量、NE样活性、IL-8和TNF-α浓度增加,α1-AT活性、TPL、DSPC/TPL和DSPC/TP下降,W/D增高.结论:急性肺损伤时中性粒细胞过度活化并释放出NE,使蛋白酶抗-蛋白酶失衡;NE对肺血管屏障具有破坏作用,促进了肺损伤的发生.
- [1]H, Taniguchi,Y, Kondoh,N, Ikuta,etc.[Diagnosis of acute respiratory distress syndrome: analysis of bronchoalveolar lavage fluid].[J].1995,33 Suppl.
- [2]Julian Diaz,Pedro L. Tornel,Pedro Jara,etc.The value of polymorphonuclear elastase in adult respiratory distress syndrome[J].1995,236(2).
- [3]G R, BARTLETT.Phosphorus assay in column chromatography.[J].1959,234.
- [4]J F, Lewis,A H, Jobe.Surfactant and the adult respiratory distress syndrome.[J].1993,147(1).
- [5]Yuizono T,Zaha M,Moriuchi H,etc.Activation of polymorphonuclear leukocytes in oleic acid-induced lung injury.[J].1998,24(7).
- [6]M, Bédard,C D, McClure,N L, Schiller,etc.Release of interleukin-8, interleukin-6, and colony-stimulating factors by upper airway epithelial cells: implications for cystic fibrosis.[J].1993,9.
- [7]谢尔凡.中性粒细胞及其释放产物在烟雾吸入伤发病机制中的作用[J].1995,(1).
- [8]Donnelly S C,MacGregor I,Zamani A,etc.Plasma elastase levels and the development of the adult respiratory distress syndrome.[J].1995.
- [9]Deng F. Liau,Nancy X. Yin,Jin Huang,etc.Effects of human polymorphonuclear leukocyte elastase upon surfactant proteins in vitro[J].1996,1302(2).
- [10]R J, Mason,J, Nellenbogen,J A, Clements.Isolation of disaturated phosphatidylcholine with osmium tetroxide.[J].1976,17.
- [11]K, Ohlsson,C B, Laurell.The disappearance of enzyme-inhibitor complexes from the circulation of man.[J].1976,51(1).
- [12].Measurement of alpha 1-antitrypsin in serum, by immunodiffusion and by enzymatic assay.[J].1974,20(3).
- [13]A I, Rivkind,J H, Siegel,P, Guadalupi,etc.Sequential patterns of eicosanoid, platelet, and neutrophil interactions in the evolution of the fulminant post-traumatic adult respiratory distress syndrome.[J].1989,210.
- [14]A B, Groeneveld,P G, Raijmakers,C E, Hack,etc.Interleukin 8-related neutrophil elastase and the severity of the adult respiratory distress syndrome.[J].1995,7(7).
- [15]Vogelmeier C,Hubbard R C,Fells G A,etc.Anti-neutrophil elastase defense of the normal human respiratory epithelial surface provided by the secretory leukoprotease inhibitor.[J].1991.
- [16]R, Buhl,A, Meyer,C, Vogelmeier.Oxidant-protease interaction in the lung. Prospects for antioxidant therapy.[J].1996,110.
- [17]Robinson-Jackson S,Xiao F,Carden D,etc.Neutrophil elastase promotes lung microvascular injury and proteolysis of endothelial cadherins.[J].1998,275(2 Pt.2).
- [18]Yasui S.,Nagai A.,Aoshiba K.,etc.A specific neutrophil elastase inhibitor (ONO-5046\u00b7Na) attenuates LPS-induced acute lung inflammation in the hamster[J].1995.
- [19]A, Lasson,K, Ohlsson.Acute pancreatitis. The correlation between clinical course, protease inhibitors, and complement and kinin activation.[J].1984,19.
- [20]J, Bieth,B, Spiess,C G, Wermuth.The synthesis and analytical use of a highly sensitive and convenient substrate of elastase.[J].1974,11(4).