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Effect and significance of pentoxifylline on pulmonary tumor necrosis factor-α mRNA expression in rabbits with traumatic acute lung injury
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 11
- DOI:
- 10.3760/j.issn:1003-0603.1999.11.001
- Key Word:
- 创伤;肺损伤,急性;己酮可可碱;基因表达;肿瘤坏死因子-α
Abstract： 目的:观察己酮可可碱(PTX)对创伤性急性肺损伤(ALI)家兔肺组织肿瘤坏死因子-α基因(TNF-α mRNA)表达的影响,探讨PTX在创伤性ALI治疗中的作用机制.方法:采用创伤性ALI模型,实验动物随机分为正常对照组(8只)、创伤性ALI模型组(24只)和PTX治疗组(24只),用ELISA和逆转录聚合酶链反应(RTPCR)方法分别检测不同时间点血浆TNF-α含量及肺组织TNF-α mRNA表达水平.结果:血浆TNF-α含量创伤性ALI模型组于创伤后1.5小时达峰值[(1.27±0.26)μg/L],肺组织TNF-α mRNA表达于创伤后8小时达高峰(2.67±0.18),二者在时间上不完全平行.PTX治疗组肺组织TNF-α mRNA的表达和外周血TNF-α含量均低于创伤性ALI模型组(P均<0.05).结论:PTX能有效抑制创伤性ALI家兔肺组织TNF-α mRNA表达;本研究为PTX治疗创伤性ALI提供了实验依据.
- [1]Remick DG.Applied molecular biology of sepsis.[J].1995,10(4).
- [2]Piotr Chomczynski,Nicoletta Sacchi.Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction[J].1987,162(1).
- [3]Redl H,Schlag G.Mediators of injury and inflammation.[J].1996,20(4).
- [4]M P, Mah,E E, Aeberhard,M B, Gilliam,etc.Effects of pentoxifylline on in vivo leukocyte function and clearance of group B streptococci from preterm rabbit lungs.[J].1993,21.