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Kinetics of serum interleukin-6,tumor necrosis factor-α,prostaglandin E2 levels and secretary phospholipase A2 activity in rats treated by lipopolysaccharide
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 9
- DOI:
- 10.3760/j.issn:1003-0603.1999.09.002
- Key Word:
- 脂多糖;细胞因子;白细胞介素-6;肿瘤坏死因子-α;前列腺素;磷脂酶A2
Abstract： 目的:研究内毒素血症大鼠血中促炎性细胞因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及花生四烯酸代谢产物前列腺素E2(PGE2)的含量和血中分泌型磷脂酶A2(sPLA2)活性产生的动态变化规律,探讨脂多糖(LPS)全面启动机体炎症反应的机制.方法:采用大鼠腹腔注射LPS复制大鼠急性内毒素血症模型,分别动态测定了正常大鼠和急性内毒素血症大鼠血清中IL-6、TNF-α、PGE2含量及sPLA2活性.结果:LPS注射后,大鼠血清中IL-6、TNF-α、PGE2含量及sPLA2活性均显著升高(与正常组比较P<0.05或P<0.01),TNF-α最先升高,在45分钟达峰值并呈双相变化,sPLA2和PGE2达峰值时间(1.5小时)晚于TNFα,并分别呈双相及三相变化;IL-6缓慢升高于6小时达峰值并呈单相变化.相关性分析显示,TNF-α含量升高与sPLA2活性升高呈正相关(r=0.819 6,P<0.05).结论:LPS可明显引发机体内的早期细胞因子反应,通过迅速激活sPLA2,促进花生四烯酸代谢,使脂类介质产生增加,可能成为感染性休克乃至多器官功能障碍综合征(MODS)发生的重要启动因素;TNF-α可能是sPLA2早期激活的关键因素.
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