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Effect of ceramide in mediating apoptosis in HL-60 cells
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 8
- DOI:
- 10.3760/j.issn:1003-0603.1999.08.006
- Key Word:
- 细胞凋亡;神经酰胺
Abstract： 目的:观察神经酰胺对HL-60细胞凋亡产生的影响、凋亡相关基因bcl2变化及与蛋白激酶C(CPK)途径的关系,探讨其在细胞凋亡过程中可能的调节作用.方法:采用DNA凝胶电泳、细胞荧光染色、流式细胞术、逆转录聚合酶链反应(RTPCR)等技术对细胞进行凋亡鉴定及bcl2mRNA表达分析.结果:外源性及内源性神经酰胺类药物可诱导HL-60细胞产生典型的凋亡改变,明显下调bcl2mRNA表达,且与刺激药物呈时间、剂量上的依赖性;CPK途径激活剂佛波酯(PMA)、二酰基甘油单独作用无诱凋作用,但加入内源性CPK抑制剂神经鞘氨醇,即可产生凋亡特有的DNA梯形降解.结论:神经酰胺类药物诱导的HL-60细胞凋亡信号,可通过调节抑凋基因bcl2表达降低而产生效应,且与CPK途径的抑制密切相关.
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